回流提取无梗五加根总木脂素的正交试验研究

研究不同提取溶剂提取无梗五加根中总木脂素的最佳工艺。方法：采用紫外-可见分光光度法，以芝麻脂素为对照品，测定样品溶液在558nm波长处的吸光度。采用正交试验法，以木脂素的含量为考察指标，对提取时间，提取次数及提取溶剂用量等影响因素进行研究。结果：对照品芝麻脂素取样量在10．6—53.0μg·ml^-1范围内吸光度与浓度呈良好的线性关系（r=0．9997）。平均回收率102．2％（RSD=1．6％，n=6）。以芝麻脂素计，无梗五加根中总木脂素含量为10．0mg·g^-1。最佳提取条件确定为无梗五加根药材粉末50％甲醇40倍量回流提取1次。结论：采用紫外分光光度法测定总木脂素含量方法简便、准确、可靠，最佳提取工艺可行。

Optimization of Reflux Extraction for Total Lignans from Acanthopanax Sessiliflorus by Orthogonal Experiment

To study the optimal process of reflux extraction for lignans from Acanthopanax sessiliflorus roots. Methods： Sesamin as the reference substance, a colorimetric method was used to detect the absorption value of the samples at 558 nm to calculate the content directly. The extraction time, the extraction times and the volume of extraction solvent were applied to optimize the extrac-tion conditions for total lignans by orthogonal experiment. Results： The absorbance had a linear relationship with the amourt of sesamin within the range of 10.6-53.0 vLg·ml^-1（ r = 0. 999 7）. The average recovery of sesamin was 102.2% （ RSD = 1.6%, n = 6）. The content of total lignans （measurement by sesamin） in the extracts was 10.0 mg·g^-1. The optimum reflux extraction conditions were as follows： adding 40-fold 50 % methanol, extracting once for 30 min. Conclusion： The UV method for determining the total lignans is feasible, stable and reliable as well as precise, and the optimal extraction process is reasonable.