| 抗人滋养层细胞表面抗原-2单抗的制备及免疫学特性分析 |
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| 引用本文： | 梁 洁,刘琼琼,张慧林,林 红,唐 奇,刘 玉,苏亦平,冯振卿,朱 进. 抗人滋养层细胞表面抗原-2单抗的制备及免疫学特性分析[J]. 南京医科大学学报(自然科学版), 2011, 0(5): 645-650 |
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| 作者姓名： | 梁 洁 刘琼琼 张慧林 林 红 唐 奇 刘 玉 苏亦平 冯振卿 朱 进 |
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| 作者单位： | 南京医科大学病理学系,卫生部抗体技术重点实验室,江苏 南京 210029;南京医科大学病理学系,卫生部抗体技术重点实验室,江苏 南京 210029;南京医科大学附属南京妇幼保健院,江苏 南京 210004;南京医科大学病理学系,卫生部抗体技术重点实验室,江苏 南京 210029;南京医科大学病理学系,卫生部抗体技术重点实验室,江苏 南京 210029;南京军区军事医学研究所,江苏 南京 210002;南京医科大学附属南京妇幼保健院,江苏 南京?摇210004;南京医科大学病理学系,卫生部抗体技术重点实验室,江苏 南京 210029;南京军区军事医学研究所,江苏 南京 210002 |
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| 基金项目： | 南京市医学科技发展重点项目（ZKX09015);南京市科技发展项目（200901083） |
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| 摘 要： | 目的:利用杂交瘤技术制备抗人滋养层细胞表面抗原-2(trophoblast cell-surface antigen 2,Trop2)单克隆抗体并鉴定其免疫学特性?方法:以胰腺癌细胞系BxPC3免疫BALB/c小鼠,取其脾细胞与SP2/0细胞融合后制备单抗?通过酶联免疫法?免疫荧光?免疫沉淀和质谱分析?流式细胞术?免疫组织化学等方法,鉴定单抗的免疫学特性?结果:通过细胞的融合与筛选,获得了持续分泌抗Trop2单抗的杂交瘤细胞株,该抗体可以识别细胞表面的Trop2膜蛋白,也可用于免疫组化识别人肿瘤组织中的Trop2蛋白,同时该抗体对乳腺癌细胞的生长具有一定的抑制作用?结论:该单抗具有作为Trop2阳性表达肿瘤靶向治疗的潜在应用价值?
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| 关 键 词： | 人滋养层细胞表面抗原-2 单克隆抗体 免疫组化 |
| 收稿时间： | 2010-12-07 |
Preparation and immunologic characterization of a monoclonal antibody against human trophoblast cell-surface antigen 2 |
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| LIANG Jie,LIU Qiong-qiong,ZHANG Hui-lin,LIN Hong,TANG Qi,LIU Yu,SU Yi-ping,FENG Zhen-qing and ZHU Jin. Preparation and immunologic characterization of a monoclonal antibody against human trophoblast cell-surface antigen 2[J]. Acta Universitatis Medicinalis Nanjing, 2011, 0(5): 645-650 |
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| Authors: | LIANG Jie LIU Qiong-qiong ZHANG Hui-lin LIN Hong TANG Qi LIU Yu SU Yi-ping FENG Zhen-qing ZHU Jin |
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| Affiliation: | Department of Pathology, Key Laboratory of Antibody Technique of Ministry of Health, NJMU, Nanjing 210029;Department of Pathology, Key Laboratory of Antibody Technique of Ministry of Health, NJMU, Nanjing 210029;Nanjing Maternity and Child Care Hospital Affiliated to NJMU, Nanjing 210004;Department of Pathology, Key Laboratory of Antibody Technique of Ministry of Health, NJMU, Nanjing 210029;Department of Pathology, Key Laboratory of Antibody Technique of Ministry of Health, NJMU, Nanjing 210029;Huadong Medical Institute of Biotechniques,Nanjing 210002,China;Nanjing Maternity and Child Care Hospital Affiliated to NJMU, Nanjing 210004;Department of Pathology, Key Laboratory of Antibody Technique of Ministry of Health, NJMU, Nanjing 210029;Huadong Medical Institute of Biotechniques,Nanjing 210002,China |
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| Abstract: | Objective:To produce a monoclonal antibody (mAb) against human trophoblast cell-surface antigen 2(Trop2) with hybridoma technology and identify its immune specificity. Methods:The spleen cells from BALB/c mice immunized with pancreatic cancer cell line BxPC3 cells were fused with SP2/0 cells to produce a monoclonal antibody against human Trop2. Further, several immunological methods such as ELISA,IF,IP,MALDI-TOF MS,FACS and IHC were used to characterize the immune specificity of the mAb. Results:The results demonstrated that the specific mouse anti-human Trop2 mAb was successfully produced and can be secreted continuously and steadily. Moreover, this mAb can effectively recognize the transmembrane glycoprotein Trop2 over-expressed in human cancer cells and tissues. Conclusion:We successfully obtained mouse anti-human Trop2 mAb which may be potentially utilized for some tumor targeted therapy. |
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| Keywords: | Trop2 monoclonal antibody immunohistochemistry |
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