血培养阳性病原菌种类及耐药性监测

目的:监测南京医科大学第一附属医院2010年血培养阳性病原菌的耐药性,指导临床合理用药?方法:收集南京医科大学第一附属医院2010年3 625份血培养阳性病原菌鉴定结果;细菌及真菌鉴定采用API系统,细菌药物敏感性测定采用纸片扩散法,真菌药物敏感性测定采用Rosco纸片法,Whonet 5.5软件进行数据分析?结果:3 625例血培养标本中有419份阳性,革兰阳性细菌占57.8%,革兰阴性细菌占38.4%,真菌占3.8%?病原菌耐药性分析结果显示:葡萄球菌和肠球菌中均未发现对万古霉素和替考拉宁的耐药株;肠杆菌科细菌对碳青霉烯类抗菌药高度敏感;非发酵菌对多种抗菌药耐药率高?结论:血培养中检出的病原菌种类较多,对多种抗菌药物的耐药率较高,应引起重视?

Analysis of the types and resistance surveillance of pathogens isolated from blood culture

Objective:To investigate resistance to pathogens isolated from blood culture of the First Affiliated Hospital of Nanjing Medical University in 2010,which can be used to guide clinical therapy. Methods: Total 3 625 blood culture samples were collected from this hospital in 2010. Bacteria and fungus were identified by API systems;bacterial drug sensitivity was determined by disk diffusion method;drug sensitivity of fungi were determined by Rosco method;and Whonet 5.5 software was used for data analysis. Results: Among the 3 625 blood culture samples,419 cases showed positive. Gram-positive bacteria accounted for 57.8%,Gram-negative bacteria accounted for 38.4%,and fungi accounted for 3.8%. The resistance analysis showed that there was no resistant strain to vancomycin and teicoplanin in Staphylococcus and Enterococcus. Enterobacteriaceae isolates were highly sensitive to carbapenem. Non-fermenting bacteria showed a relatively high resistance to many antimicrobial drugs. Conclusion: There are many different kinds of pathogens isolated from blood culture,which showed high resistance to many antimicrobial agents.