| sunitinib对人气道平滑肌细胞增殖、迁移和ERK磷酸化的影响 |
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| 引用本文： | 刘 媛,黄 茂,李 涛,刘 红.sunitinib对人气道平滑肌细胞增殖、迁移和ERK磷酸化的影响[J].南京医科大学学报,2011(6):803-808. |
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| 作者姓名： | 刘 媛 黄 茂 李 涛 刘 红 |
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| 作者单位： | 南京医科大学第一附属医院呼吸科,江苏 南京 210029;南京医科大学第一附属医院呼吸科,江苏 南京 210029;南京医科大学第一附属医院呼吸科,江苏 南京 210029;南京医科大学第一附属医院呼吸科,江苏 南京 210029 |
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| 摘 要： | 目的:探讨sunitinib对血小板源性生长因子(platelet-derived growthfactor,PDGF)-BB刺激的人气道平滑肌细胞(human airway smooth muscle cells,HASMCs)增殖和迁移的影响,及其对细胞外信号调节激酶(extracellular signal re...
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| 关 键 词： | 人气道平滑肌细胞 sunitinib 血小板源性生长因子-BB U0126 细胞外信号调节激酶 |
| 收稿时间： | 2010/12/13 0:00:00 |
The effect of sunitinib on the proliferation, migration and phosphorylation of ERK of human airway smooth cell |
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| LIU Yuan,HUANG Mao,LI Tao and LIU Hong.The effect of sunitinib on the proliferation, migration and phosphorylation of ERK of human airway smooth cell[J].Acta Universitatis Medicinalis Nanjing,2011(6):803-808. |
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| Authors: | LIU Yuan HUANG Mao LI Tao and LIU Hong |
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| Institution: | Department of Respiration,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Respiration,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Respiration,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Respiration,the First Affiliated Hospital of NJMU,Nanjing 210029,China |
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| Abstract: | Objective:To investigate the effect of sunitinib on platelet-derived growth factor(PDGF)induced human airway smooth muscle cells(HASMCs) proliferation, migration and phosphorylation of ERK(p-ERK). Methods: HASMCs derived from human bronchus were divided into six groups: normal group, PDGF-BB group, PDGF-BB and sunitinib intervention group, sunitinib intervention group, U0126 intervention group, PDGF-BB and U0126 intervention group. Cell cycle progression was analyzed by flow cytometry. Migration was examined by Transwell test. The protein expressions of p-ERK were determined by Western blot analysis. Results: PDGF-BB (20 ng/ml) increased the proliferation and migration of HASMCs significantly (P < 0.01); sunitinib(3 nmol/L) decreased the proliferation and migration of HASMCs stimulated by PDGF-BB significantly(P < 0.05), which was no difference compared with U0126. The expression of p-ERK in PDGF-BB group was higher than that of the normal group(P < 0.01), which can be decreased by sunitinib. There is no difference between the effect of sunitinib and U0126 on p-ERK expression induced by PDGF-BB. Conclusion: Sunitinib can directly inhibit HASMCs proliferation and migration induced by PDGF-BB through the modulation of the ERK pathway. |
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| Keywords: | human airway smooth cells sunitinib PDGF-BB U0126 ERK |
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