| 锌指蛋白ZBED3在小鼠卵细胞中的表达及意义 |
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| 引用本文： | 崔 婷,张 平,郭雪江,霍 然,毕 晔. 锌指蛋白ZBED3在小鼠卵细胞中的表达及意义[J]. 南京医科大学学报(自然科学版), 2011, 0(9): 1274-1276 |
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| 作者姓名： | 崔 婷 张 平 郭雪江 霍 然 毕 晔 |
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| 作者单位： | 南京医科大学第一附属医院检验科,江苏 南京 210029;南京医科大学组织胚胎学系,江苏 南京 210029;南京医科大学第一附属医院检验科,江苏 南京 210029;南京医科大学组织胚胎学系,江苏 南京 210029;南京医科大学组织胚胎学系,江苏 南京 210029 |
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| 基金项目： | 国家自然科学基金青年科学基金(31100842) |
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| 摘 要： | 目的:研究锌指蛋白(zinc finger,BED-type containing 3,ZBED3)在小鼠卵母细胞中的表达及分布,探讨ZBED3在小鼠卵细胞成熟及早期胚胎发育过程的功能?方法:运用生物信息学资源分析ZBED3在多组织中的表达情况,运用免疫印迹法和免疫组织化学术,检测ZBED3在小鼠卵细胞中的表达和分布?结果:生物信息学检索显示ZBED3 mRNA在卵细胞及受精卵中特异性表达,免疫印迹法证实ZBED3在小鼠卵母细胞中高表达,同时免疫组织化学显示阳性信号主要集中在卵母细胞胞浆中?结论:ZBED3可能在卵母细胞成熟和早期胚胎发育过程中发挥作用?
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| 关 键 词： | ZBED3 小鼠 卵母细胞 |
| 收稿时间： | 2011-05-10 |
The expression and significance of Zinc finger protein ZBED3 in mouse oocyte |
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| CUI Ting,ZHANG Ping,GUO Xue-jiang,HUO Ran and BI Ye. The expression and significance of Zinc finger protein ZBED3 in mouse oocyte[J]. Acta Universitatis Medicinalis Nanjing, 2011, 0(9): 1274-1276 |
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| Authors: | CUI Ting ZHANG Ping GUO Xue-jiang HUO Ran BI Ye |
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| Affiliation: | Clinical Laboratory,the First Affiliated Hospital of NJMU,Nanjing 210029;Department of Histology and Embryology,NJMU,Nanjing 210029,China;Clinical Laboratory,the First Affiliated Hospital of NJMU,Nanjing 210029;Department of Histology and Embryology,NJMU,Nanjing 210029,China;Department of Histology and Embryology,NJMU,Nanjing 210029,China |
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| Abstract: | Objective: To study the expression and distribution of ZBED3(zinc finger,BED-type containing 3) in mouse oocyte,and explore its role in oocyte maturation and early embryo development. Methods: The expressional level of ZBED3 mRNA in multipule tissues was analyzed using bioinformatic resources. Western blot and immunohistochemistry were used to detect the expression and distribution of ZBED3 in mouse oocyte. Results: The result of bioinformatic analysis showed that ZBED3 mRNA was specificly expressed in mouse oocyte and fertilized egg. Western blot confirmed the high-level expression of ZBED3 protein in mouse oocyte. And the result of immunohistochemistry showed the positive signal of ZBED3 was centralized in the cytoplasm of oocyte. Conclusion: ZBED3 maybe play roles in oocyte maturation and early embryo development. |
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| Keywords: | ZBED3 mouse oocyte |
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