麋鹿角醇提液对环磷酰胺致雄鼠性腺损伤的保护作用

目的:观察麋鹿角醇提液对环磷酰胺所致雄性小鼠性腺损伤的保护作用?方法:应用腹腔注射环磷酰胺造成小鼠性腺抑制模型,给予麋鹿角醇提液不同剂量进行干预,然后测定小鼠血清中的睾酮以及黄体生成素的水平,检测睾丸以及副性器官质量指数?精子密度和精子活动率,检测睾丸组织中超氧化物歧化酶(superoxide dismutase,SOD) 和丙二醛(malonaldehyde,MDA)含量,光镜检测睾丸组织形态学变化?结果:麋鹿角醇提液能良好对抗环磷酰胺引起的睾酮与黄体生成素下降,能提高模型睾丸质量指数,提高模型精子密度与精子活率,增加模型组睾丸SOD活力,并减少其MDA含量,改善环磷酰胺所致模型小鼠睾丸组织的病理性变化?结论:麋鹿角醇提液对环磷酰胺致雄鼠性腺损伤具有保护作用,可能与其促性激素样作用以及抗氧化作用有关?

Protection of Elk antlers ethanolic fluidextract on gonad dysfunction impairment of male mice induced by cyclophosphamide

Objective: To observe the protective effect of Elk antlers ethanolic fluidextract(EAEF) on gonad function impairment of male mice induced by cyclophosphamide(CP). Methods: The model of gonad function depression was prepared by repeatedly intraperitoneal injection of CP. Low and high dose of EAEF were given to mice with oral administration to intervene this process,then the level of testosterone and luteinizing hormone in serum were detected;the weight index of sex organ and accessory sex organ were observed;sperm density and sperm activity were detected;the superoxide dismutase (SOD) activity and malonaldehyde(MDA) content were measured. Meanwhile the histopathological changes in testis were observed by light microscopy. Results: EAEF reversed the decline of testosterone and luteinizing hormone level in serum,enhanced the testicle mass index,sperm density and sperm activity,increased SOD activity and decreased content of MDA in testis,as well alleviated the histologic pathological formation in testis in cyclophosphamide-induced model mice. Conclusion: EAEF has protective effect on gonad dysfunction impairment of male mice induced by CP,which might be related to its the gonadotropin hormone-like effect and antioxidation.