| 萘哌地尔抗大鼠血管平滑肌细胞增殖的作用 |
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| 引用本文： | 李利生,孙安盛,余丽梅,吴芹,黄燮南,袁牧.萘哌地尔抗大鼠血管平滑肌细胞增殖的作用[J].中国药理学通报,2008,24(12). |
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| 作者姓名： | 李利生 孙安盛 余丽梅 吴芹 黄燮南 袁牧 |
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| 作者单位： | 1. 遵义医学院药理学教研室,贵州,遵义,563003
2. 贵州省中国科学院天然产物化学重点实验室,贵州,贵阳,550002 |
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| 基金项目： | 国家自然科学基金资助项目
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| 摘 要： | 目的研究萘哌地尔(naftopidil,Naf)对去甲肾上腺素(noradrenalin,NA)诱导大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响并探讨其机制。方法培养的VSMCs经NA诱导其增殖,分别用MTT比色法和细胞计数法检测Naf对VSMCs增殖的影响;荧光法测定细胞内钙浓度(Ca2+]i)及Real-TimeRT-PCR检测c-myc、c-fos、高血压相关基因(hypertension related gene-1,HRG-1)mRNA的表达。结果萘哌地尔(10-7～10-6mol·L-1)能够抑制NA诱导的VSMCs增殖,并明显降低VSMCsCa2+]i,降低c-myc、c-fos mRNA表达,增加HRG-1 mRNA的表达。结论萘哌地尔明显抑制NA诱导的VSMCs增殖,作用机制可能与其降低VSMCsCa2+]i、拮抗NA上调c-myc、c-fos mRNA的表达和拮抗NA下调HRG-1 mRNA的表达有关。
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| 关 键 词： | 萘哌地尔 血管平滑肌细胞 去甲肾上腺素 增殖 |
The antiproliferation effect of naftopidil in vascular smooth muscle cells of rats |
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| LI Li-sheng,SUN An-sheng,YU Li-mei,WU Qin,HUANG Xie-nan,YUAN Mu.The antiproliferation effect of naftopidil in vascular smooth muscle cells of rats[J].Chinese Pharmacological Bulletin,2008,24(12). |
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| Authors: | LI Li-sheng SUN An-sheng YU Li-mei WU Qin HUANG Xie-nan YUAN Mu |
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| Institution: | LI Li-sheng1,SUN An-sheng1,YU Li-mei1,WU Qin1,HUANG Xie-nan1,YUAN Mu2(1.Dept of Pharmacology,Zunyi Medical College,Zunyi Guizhou 563003,China,2. The Key Laboratory of Chemistry forNatural Produces of Guizhou Province , Chinese Academy Sciences,Guiyang 550002,China) |
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| Abstract: | Aim To study the effect of naftopidil(Naf) on noradrenalin(NA)-induced proliferation of rats vascular smooth muscle cells (VSMCs),and explore its mechanisms. Methods The cultured VSMCs was induced to proliferate by NA,and effects of Naf on the VSMCs proliferation was tested by MTT assay and cell counting method respectively. The intra-cellular calcium concentration is investigated by fluorimetry,and the expressions of c-myc,c-fos and hypertension related gene-1(HRG-1) mRNA were tested by Real-Time RT-PCR. R... |
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| Keywords: | naftopidil vascular smooth muscle cells noradrenalin proliferation |
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