Development and application of a urodilatin (CDD/ANP-95#x2013;126)-specific radioimmunoassay |
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Authors: | Christian Drummer Fritz Fiedler Achim Bub Dagmar Kleefeld Eve Dimitriades Rupert Gerzer Wolf-Georg Forssmann |
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Institution: | (1) Medizinische Klinik, Klinikum Innenstadt der Universität, Ziemssenstrasse 1, W-8000 München 2, Germany;(2) Niedersächsisches Institut für Peptid-Forschung (IPF), Feodor-Lynen-Strasse 31, W-3000 Hannover 61, Germany;(3) Institut für Flugmedizin, Deutsche Forschungsanstalt für Luft- und Raumfahrt (DLR), Linder Höhe, W-5000 Köln 90, Germany |
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Abstract: | Urodilatin, a renal natriuretic peptide that is an analogue to circulating atrial natriuretic peptide -ANP (99-126)], is measurable with a highly specific and sensitive radioimmunoassay. While most ANP antibodies cannot distinguish between urodilatin and other ANP analogues, the polyclonal urodilatin antibody specifically measures human urodilatin without any cross-reactivity to other ANP analogues. Urodilatin is not detected in blood from healthy volunteers nor from cardiac patients. Urinary urodilatin accounts for only a part of total urinary ANP immunoreactivity. Urodilatin excretion closely parallels sodium excretion in response to an acute volume load while changes in urinary immunoreactive ANP excretion do not reflect this renal response. We conclude that specific urodilatin assays are required to explore further the physiological role of the renal natriuretic peptide. |
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Keywords: | Atrial natriuretic peptide Kidney Renal natriuretic peptide Sodium excretion Urodilatin |
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