p53、rasp21及C-myc癌基因蛋白在胃癌中的表达及分布特征

目的通过对胃癌标本中p53、rasp21及C-myc癌基因蛋白的表达分析，探讨其在胃癌发生中的作用。方法用免疫组化方法检测了60例胃癌标本中p53、rasp21及C-myc的表达及分布。结果p53、rasp21及C-myc在胃癌中的阳性表达分别为51.7％、40.0％、66．7％；胃癌旁正常黏膜七皮的阳性率分别为1．6％、13．3％、15．0％，与肿瘤区相比较有显著差异（P〈0．01）。rasp21及Cmyc在肿瘤区与肿瘤移行区、癌旁正常黏膜区染色强度比较有差异（P〈0．05）。p53及C-myc阳性表达与肿瘤的分化程度有关，即分化越差，其阳性表达越高。结论p53、rasp21及C-myc与胃癌的发生、发展密切相关，p53及Gmyc在肿瘤分化过程中可能起重要作用。

Expression and distribution feature of p53, Rasp21 and C-myc oncoproteins in carcinoma of stomach

Objective To investigate the role of p53, rasp21 and C-myc in gastric carcinogenesis. Methods Expression and distribution feature of p53, rasp21 and C-myc in 60 cases of human gastric cancer were detected with immunohistochemical SP (Streptavidin-Peroxidase) staining. Results The positive rate of expression was respectively 51.7% for p53, 40.0% for rasp21, and 66.7% for C-myc, while in the gastric mucous membrane adjacent to carcinoma they were respectively 1.6% for p53, 13.3% for rasp21, and 15.0% for C-myc. The differences in expression of p53, rasp21 and C-myc oncoproteins in carcinoma and non-carcinoma tissues were very significant (P<0.01), and there was significant difference (P<0.05) in the density of rasp21 and C-myc staining between tumor region, translated region, and adjacent mucous membrane. The positive expression of p53 and C-myc was related to the differentiation of tumor tissues, the poorer the differentiation, the higher the positive expression. Conclusion p53, rasp21 and C-myc were closely correlated with pathogenesis and development of gastric carcinoma, and the expression of p53 and C-myc may contribute to the degree of tumor differentiation.