Rg1对环孢素A慢性肾毒性大鼠肾脏肾小管上皮-间充质细胞转分化的抑制作用

目的探讨人参皂苷Rg1(G-Rg1)对环孢素A(CsA)慢性肾毒性大鼠肾脏肾小管上皮-间充质细胞转分化(EMT)的抑制作用。方法雄性SD大鼠24只,随机分为3组:正常组、模型组、G-Rg1干预组,采用低盐饮食加CsA灌胃的方法建立CsA慢性肾纤维化模型,CsA剂量为25 mg·kg-1·d-1。观察G-Rg1对大鼠尿量、肾功能、肾脏病理及肾脏转化生长因子(TGF)-β1、E-钙黏素、α-平滑肌肌动蛋白(SMA)表达的影响。结果 G-Rg1能抑制CsA慢性肾毒性大鼠肾脏纤维化,下调TGF-β1、α-SMA在肾组织的表达,同时上调肾组织E-钙黏素的表达。结论 G-Rg1对CsA慢性肾毒性大鼠具有保护作用,其机制可能是抑制了肾组织的EMT途径。

Rgl attenuates epithelial-mesenchymal transformation in rats with chronic cyclosporine A nephrotoxicity

Objective To investigate the effects of ginsenoside Rgl （G-Rgl） on renal epithelial-mesenchymal transformation （EMT） in rats with chronic cyclosporine A （CsA） nephrotoxicity. Methods Twenty-four male SD rats were randomly assigned to normal control group, model control group and G-Rgl treatment group. Low-salt diet and CsA were administered by gastric injection at the dose of 25mg·kg^-1·d^-1 for establishing the models of chronic CsA nephrotoxicity. The effects of G-Rgl on the volume of urine, renal function, renal pathophysioiogy and renal TGF-β1 E-cadherin and α-SMA expressions were determined. Results G-Rgl down-regulated TGF-β1 and α-SMA and upregulated E-cadherin expressions in renal tissues in rats with chronic CsA nephmtoxicity. Conclusion G-Rgl harbors protective effects to the kidneys in rats possibly via inhibition of the EMT process.