细胞外信号调节激酶在缺糖缺氧/复糖复氧神经元中的表达与银杏叶提取物的调节作用

目的研究细胞外信号调节激酶(ERK1/2)在缺糖缺氧/复糖复氧神经元中的表达以及银杏叶提取物的调节作用。方法利用原代培养的皮层神经元,通过去除培养液中的糖和氧(oxygen and glucose deprivation,OGD)模拟缺血缺氧,恢复糖氧供给模拟再灌注。通过免疫蛋白印迹法测定ERK1/2、p-ERK1/2蛋白的表达。再灌注时加用银杏叶提取物(EGB761)观察其对神经元保护作用以及时ERK1/2表达的调节作用。结果缺糖缺氧/复糖复氧后神经元p-ERK1/2表达明显下降,EGB761可剂量依赖地保护神经元活性,并可上调p-ERK1/2蛋白的表达。结论EGB761对培养的神经元缺糖缺氧/复糖复氧损伤有保护作用,该作用可能与激活ERK信号转导通路有关。

Expression of extracellular signal - regulated kinase in cultured reperfusion neurons and the role of EGB761

Objective To investigate the expression of extracellular signal-regulated kinase in cultured reperfusion neurons and the effects of EGB761.Methods The ischemic-reperfusion model was established by deprivating both glucose and oxygen in medium,then gave them again.Western blotting was used to detect the expression of both ERK1/2 and p-ERK1/2 protein.EGB761 was added with 3 different concentrations at the beginning of reperfusion.Result The expression of p-ERK1/2 was downragulated after ischemia-reperfusion injury.When treating with EGB761,the reduction of both MTT and expression of p-ERK1/2 protein was reversed.Conclusion EGB761 could protect the neurons against reperfusion injury,and the mechanism of this effect may be related to alerting p-ERK1/2 protein expression.