HIV/AIDS患者疾病进程与CD4+CD25hi调节性T细胞之间相关性研究

目的 通过分析不同阶段HIV感染者外周血CD4+CD25hi调节性T细胞(CD4+CD25hiregulatory T cells,Treg cells)与外周血免疫状态和病毒载量的相关性,探讨Treg细胞对HIV/AIDS发病进程的影响.方法 采集116例HIV感染者和21例正常人对照外周血,用4色流式细胞术进行CD4+和CD8+T细胞绝对数计数;用3色流式细胞术进行Treg细胞测定;用荧光定量PCR法进行HIVRNA载晕测定.实验数据用回归统计学方法和T检验方法进行分析.结果 HIV感染者外周血Treg细胞频率在HIV感染初期显著下降,之后随着疾病的进程逐渐升高.在CD4+T细胞大于300/μl的患者低于正常对照组,在CD4+T细胞小于100/μl的患者高于正常对照组,差异具有统计学意义.Treg细胞频率与CD4+T淋巴细胞绝对数和CD4+/CD8+之间均呈负相关.其相关系数r和P值各为r=-0.564,P＜0.001和r=-0.377,P＜0.001;Treg细胞频率与血浆HIV病毒载量呈正相关,其相关系数r=0.514.P＜0.001.结论 CD4+CD25hi Treg细胞可能是参与艾滋病免疫发病机理的重要细胞,在HIV感染发病进程的不同阶段具有不同的意义,其确切机制有待进行进一步研究.

Relationship of CD4+ CD25hi Regulatory T(Treg)Cells to Disease Progression in HIV-infected Patients

Objective Analyzing the relationships between peripheral blood CD4+ CD25hi regulatory T (Treg)cells and peripheral blood immune status or plasma HIV-1 viral load in HIV-infected individuMs,so as to determine whether Treg were related to the progression of HIV-infected disease.Methods116 HIV-infected patients in different stages and 21 healthy control individuals were included in this study.The CD4+ and CD8+ T cell counts were determined by a standard 4-color flow cytometry technique.The Treg cells were examined with 3-color immune staining flow cytometry.The plasma HIV-1 viral load was detected by real time PCR.Results The frequencies of Treg cells decreased in HIV-infected individuals with high CD4+ T cell counts(＞300/μl)compared with normal controls.With the progression of disease the frequencies of Treg cells were raised gradually,until were increased in HIV-infected individuals with low levels of CD4+ T cell counts(＜100/μl).In addition,the frequencies of Treg cells were inversely related to CD4+ T cell counts and CD4+/CD8+ ratio,data showed a statistically significant(respectively,r=-0.564,P＜0.001;r=-0.377,P＜0.001).Furthermore,the proportions of Treg cells were closely related to plasma HIV-1 RNA viral load(r=0.514,P＜0.001).Conclusion CD4+ CD25hi Treg cells should be a kind of important cells participating the immunopathogenesis of AIDS.It may play different roles in different stages of HIV-infected disease.The exact mechanism of Treg cells in the progression of the HIV-infected disease needs to be investigate further.