重组人白细胞介素-13刺激Dami细胞增殖

目的和方法：研究重组人白细胞介素－１３（rhＩＬ－１３）对Ｄami细胞的作用，在血浆 凝决培养基中分别加入rhＩＬ－１３５０ng/mＬ、１００ng/mＬ、１５０ng/mＬ，计数Ｄami细胞ＤＮＡ中的５－溴－２－脱氧尿嘧啶（５－ＢrdＵ）。结果：血浆凝块培养基中加入rhＩＬ－１３能ami使细胞扩增，５０ng/mＬ、１００ng/mＬ、１５０ng/mＬ rhＩＬ－１３ 实验组Ｄami细胞集落数分别是对照组的１．４２倍、１．８５倍、２．３１倍。５－Ｂrd

Dami cell proliferation stimulated by recombinant human interleukin - 13

AIM and METHOD:To study the effect of recombinant human interleukin-13(rhIL-13) on Dami cells, rhIL-13, at concentrations of 50 ng/mL, 100 ng/mL and 150 ng/mL, was added to plasma clot culture for counting of Dami cell colonies and to liquid medium for detecting incorporation of 5-bromo-2'-deoxy-uridine (5-BrdU) into Dami cell DNA. RESULTS: rhIL-13 added to plasma clot cultures resulted in Dami cell expansion. The colony numbers of Dami cells treated with rhIL-13 50 ng/mL, 100 ng/mL and 150ng/mL were 1.42 times, 1.85 times and 2.31 times greater than those in the controls, respectively. The findings in 5-BrdU assay showed that the absorbance values of the experimental groups treated with 50 ng/mL, 100 ng/mL and 150 ng/mL of rhIL-13 were 47.06%, 88.24% and 114.71% higher than those of the controls, respectively. CONCLUSION: rhIL-13 enhances proliferation of Dami cells.