红景天对乳腺癌治疗的研究

目的：研究红景天提取物对乳腺癌MDA-MB-435细胞株增殖及血管新生相关因子的影响。方法：人乳腺癌MDA-MB-435细胞培养后，加入红景天提取物10mg／ml、20mg／ml，设置空白对照组。药物治疗24小时后，使用流式细胞仪进行分选、MTT法测定细胞增殖能力，以及VEGF表达的影响。结果：红景天处理后，MDA-MB-435细胞株的形态学特征发生显著变化，且随着药物浓度增加而愈加明显；阻滞MDA-MB-435细胞增殖周期的作用，主要阻滞于S期，该作用随着药物浓度增加亦增强；显著抑制MDA-MB-435细胞的增殖活性：处理24小时，各剂量组肿瘤细胞表达VEGF mRNA无明显差异，44小时后，给药组明显地抑制MDA-MB-435细胞表达VEGF的作用。结论：红景天提取物在体外可阻滞乳腺癌MDA-MB-435细胞增殖周期、降低乳腺癌MDA-MB-435细胞的增殖活性、减少促血管新生因子VEGF表达。

Study on the Therapy of Integripetal Rhodiola Herb to Breast Cancer

To research effect of Integripetal Rhodiola Herb extractive to breast cancer MDA-MB-435 cell and VEGF. Methods MDA-MB-435 cell cultured, then added 10mg/ml and 20mg/ml of Integripetal Rhodiola Herb extractive. After 24 hr treatment, sorted with flow cytometry, and detected cell proliferation bu MTT method, and VEGF exprssion. Results After treated by Rhodiola extract for 24hours, the morphological feature of MDA-MB-435 cells had changed significandy with the increase of drug concentration; Rhodiola treatment groups showed S-stage arrest and the blocking was more significant with higher drug concentration; Rhodiola extract clearly inhibited proliferation of human breast cancer cell line, MDA-MB-435, and inhibition was more significant with time and the dose increase; After Rhodiola extract intervention given to human breast cancer cell MDA-MB-435 for 24h, there was not difference of VEGF expression among all groups; after 44h treatment, the VEGF rnRNA levels were down-regulated in Rhodiola group, and there was obviously different between Rhodiola groups and control group. Conclusion In vitro studies, Rhodiola could inhibit the proliferation of human breast cancer MDA-MB-435 cells, reduce the expression of VEGF.