Transient retinal ischemia-reperfusion in rats |
| |
Authors: | ZHOU Yuehua Luther E.Lindner George C.Y.Chiou |
| |
Affiliation: | Department of Ophthalmology, Tongren Hospital, Beijing 100730, China;Department of Pathology and Laboratory Medicine, Texas A&M University College of Medicine, College Station, Texas 77843-1114, USA;Institute of Ocular Pharmacology and Department of Pharmacology & Toxicology, Texas A&M University College of Medicine, College Station, Texas 77843-1114, USA;Department of Pathology and Laboratory Medicine, Texas A&M University College of Medicine, College Station, Texas 77843-1114, USA;Department of Ophthalmology, Tongren Hospital, Beijing 100730, China |
| |
Abstract: | OBJECTIVE: To investigate the effect of transient ischemia-reperfusion on the retina in rats. METHODS: Retinal ischemia-reperfusion was induced in rats by increasing the intraocular pressure. After 1 or 5 minutes of ischemia, retinal neuronal cell death at different periods of reperfusion was studied using the TdT-deoxynucleotide terminal nick-end labeling (TUNEL) method and light microscopy. Retinal IL-1 beta and TNF alpha were quantified by an enzyme-linked immunosorbent assay (ELISA). RESULTS: A few migrating leukocytes were noticed in the retina after transient retinal ischemia-reperfusion. Rare TUNEL-positive (T+) cells were noticed in the outer granular layer or the rod and cone layer, and not in ganglion cell layer in control eyes, but they were significantly increased in the outer granular layer, the inner granular layer, and ganglion cell layer in the eyes treated with 1 or 5 minutes of retinal ischemia-reperfusion (P < 0.05). Retinal IL-1 beta was significantly increased at 6 hours after reperfusion in the eyes treated with 1 or 5 minutes ischemia over the control eyes (P < 0.05), but retinal TNF alpha was not significantly increased (P > 0.05). CONCLUSION: Transient retinal ischemia-reperfusion for only 1 or 5 minutes of ischemia can induce the upregulation of retinal IL-1 beta and apoptosis of retinal neuronal cells. This kind of apoptosis in individual cells, however, was not sufficient to affect the whole retinal function. |
| |
Keywords: | retina ischemia-reperfusion apoptosis interleukin-1 tumor necrosis factor |
本文献已被 CNKI 万方数据 等数据库收录! |
| 点击此处可从《中华医学杂志(英文版)》浏览原始摘要信息 |
|
点击此处可从《中华医学杂志(英文版)》下载全文 |
|