| 穿心莲指纹图谱和不同产地质量研究 |
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| 引用本文： | 李军,段然,黄雯,李卓明,毕文川,陈小莺,董婷霞,詹华强.穿心莲指纹图谱和不同产地质量研究[J].中国现代中药,2009,11(6):21-24. |
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| 作者姓名： | 李军 段然 黄雯 李卓明 毕文川 陈小莺 董婷霞 詹华强 |
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| 作者单位： | 香港科技大学生物系及中药研发中心,香港 |
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| 摘 要： | 目的:建立穿心莲药材有效的质量评价方法.方法:通过建立HPLC指纹图谱和有效成分的含量测定,对来自10个不同产地的穿心莲药材进行分析.色谱条件为Alltima C18色谱柱(4.6m×250mm, 5μm),流动相为乙腈-水系统,梯度洗脱,流速1.0mL·min-1,指纹图谱检测波长为215nm,穿心莲内酯和脱水穿心莲内酯含量测定的检测波长分别为225nm和254nm.结果:得到分离度、重现性均较好的穿心莲药材HPLC指纹图谱,标示了10个共有色谱峰,对10批来自不同产地穿心莲样品的色谱指纹图谱进行了相似度评价;应用HPLC-DAD测定了不同产地穿心莲样品中穿心莲内酯和脱水穿心莲内酯的含量,结果显示,穿心莲药材中穿心莲内酯和脱水穿心莲内酯总量介于1.09%～4.47%.结论:该方法简便准确,灵敏度高、重复性好,可以作为穿心莲药材的质量控制方法,此方法已被<香港中药材标准>采用.建议HPLC指纹图谱用于穿心莲药材的质量控制,穿心莲内酯和脱水穿心莲内酯的总量不得低于1.0%.
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| 关 键 词： | 质量评价 穿心莲 HPLC指纹图谱 含量测定 |
| 收稿时间： | 8/8/2008 12:00:00 AM |
Study on the Fingerprint and Quality of Herba Andrographidis from Different Habitats |
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| Li Jun,Duan Ran,Huang Wen,Li Zhuoming,Bi Wenchuan,Chen Xiaoying,Dong Tingxia.Study on the Fingerprint and Quality of Herba Andrographidis from Different Habitats[J].Modern Chinese Medicine,2009,11(6):21-24. |
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| Authors: | Li Jun Duan Ran Huang Wen Li Zhuoming Bi Wenchuan Chen Xiaoying Dong Tingxia |
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| Institution: | Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China;Center for Chinese Medicine R & D and Department of Biology, The Hong Kong Universityof Science and Technology, Hong Kong, China |
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| Abstract: | |
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| Keywords: | Quality assessment Herba Andrographis HPLC fingerprint Content determination |
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