CpG ODN纳米粒增强免疫抑制小鼠对重组乙肝疫苗的免疫应答

目的：探讨CpG ODN壳聚糖纳米粒联合重组乙肝疫苗对免疫抑制小鼠的免疫增强作用。方法：选月环磷酰胺建立免疫抑制模型小鼠。将乙肝疫苗单独或和CpG ODN或CpG ODN纳米粒经后腿胫骨前肌注射到小鼠体内，ELISA方法检测抗HBsAg IgG抗体、IL-12水平；流式细胞仪检测外周血CD4^＋、CD8^＋T淋巴细胞亚群。结果：CpG ODN纳米粒联合疫苗组的CD4^＋细胞百分数，IL-12水平及抗HBsAg IgG抗体的含量显著高于CpG ODN联合疫苗组（P〈0．05）。结论：包裹在壳聚糖纳米粒中的CpG ODN较相同剂量的CpG ODN更能增强免疫抑制小鼠对乙肝疫苗的免疫应答。

Enhance of Immune Responses of Chitosan-CpG ODN Nanoparticles in Immunodepressed Mice with Hepatitis B Vaccine

Objective:To investigate the immune responses of synthetic CpG ODN by preparing chitosan nanoparticles in immu- nodepressed mice to recombinant hepatitis B vaccine.Method:The model of immunity suppression mice was established by CTX.The same doses of hepatitis B vaccine with or without CpG ODN or CpG ODN nanoparticles were injected into the left tibialis anterior mus- cle of all the immune mice.The serum anti-HBsAg IgG and IL-12 levels in mice were measured by ELISA assays.Changes of peripher- al blood T lymphocyte subsets CD4~+,CD8~+ were detected by flow cytometry.Result:Percentages of CD4~+,IL-12 levels,and anti- HBsAg IgG level of mice immunized with hepatitis B vaccine plus CpG ODN nanoparticles increased more than the mice injected by hepatitis B vaccine plus CpG ODN.Conclusion:These studies emphasize that Chitosan-CpG ODN delivery in biodegradable nanoparti- cles combining with hepatitis B vaccine can facilitate the immune response in immunodepressed mice at same doses of CpG ODN.