hsa-miR-23a-3p在肺腺癌组织中的表达及意义

目的]探讨hsa-miR-23a-3p在肺腺癌织中的表达水平及临床意义。方法]收集我院呼吸科就诊的42例肺腺癌患者的癌组织及癌旁组织样本,同时选取37例非肺腺癌患者的肺穿刺样本为对照组。采用荧光定量PCR法检测各组患者肺组织中hsa-miR-23a-3p相对表达水平,分析hsa-miR-23a-3p在肺腺癌组织中的表达及与患者临床特征的关系;以患者血清癌胚抗原(CEA)指标为参考,利用受试者工作特征曲线(ROC)评估肺腺癌患者癌组织中hsa-miR-23a-3的表达临床应用价值。结果]对照组与癌旁组织中hsa-miR-23a-3p的表达水平差异无统计学意义(4.32±0.34 vs 4.16±0.41,P>0.05),与对照组与癌旁组织比,肺腺癌组织hsa-miR-23a-3p表达较高(6.08±0.47 vs 4.32±0.34,6.08±0.47 vs 4.16±0.41,P<0.01)。不同性别(6.16±0.82 vs 5.89±0.73)、分化程度(6.20±0.84 vs 5.97±0.67)癌组织的hsa-miR-23a-3p表达差异无统计学意义(P>0.05),TNM分期越高(5.13±0.49 vs 6.26±0.51)、肿瘤越大(6.47±0.65 vs 5.94±0.71)、淋巴结转移(6.32±0.53 vs 5.83±0.46)及CEA阳性(6.47±0.44 vs 5.79±0.41)癌组织的hsa-miR-23a-3p表达显著性增高(P<0.001)。ROC曲线分析显示,与CEA比,hsa-miR-23a-3p的曲线下面积高(0.779vs 0.683)。结论]肺腺癌组织hsa-miR-23a-3p呈高表达,与淋巴结转移、肿瘤大小及TNM分期相关,可用于辅助诊断肺腺癌。

Expression and Clinical Significance of hsa-miR-23a-3p in Lung Adenocarcinoma

Objective] To investigate the expression and clinical significance of hsa-miR-23a-3p in lung adenocarcinoma. Methods] The cancer tissue samples and paracancerous tissue samples of 42 patients with lung adenocarcinoma admitted to our hospital were collected. The lung puncture samples of 37 patients with non-lung adenocarcinoma were obtained as the control group. The expression of hsa-miR-23a-3p in tissue samples was detected by RT-PCR. The relationship between the expression of hsa-miR-23a-3p in lung adenocarcinoma and clinical characteristics of patients was analyzed. The serum carcinoembryonic antigen(CEA) levels of patients were measured. The receiver operating characteristic(ROC) was applied to evaluate the value of hsa-miR-23a-3p in diagnosis of lung adenocarcinoma. Results] There were no significant differences in the expression level of hsa-miR-23a-3p between the control group and the paracancerous tissues(4.32±0.34 vs 4.16±0.41,P>0.05). Compared with the control group and paracancerous tissues,the expression of hsa-miR-23a-3p in lung adenocarcinoma tissue was higher(6.08 ±0.47 vs 4.32 ±0.34,6.08 ±0.47 vs4.16±0.41,P<0.05). There was no significant difference in the expression of hsa-miR-23a-3p between male and female patients(6.16±0.82 vs 5.89±0.73) or patients with different differentiation of cancer tissue(6.20±0.84 vs 5.97±0.67)(P>0.05). The patients with higher TNM stage(5.13±0.49 vs 6.26±0.51),larger tumor size(6.47±0.65 vs 5.94±0.71),positive lymph node metastasis(6.32±0.53 vs 5.83±0.46),or positive serum CEA level(6.47±0.44 vs 5.79±0.41) had higher expression of hsa-miR-23a-3p(all P<0.05). ROC curve analysis showed that the area under the curve(0.779 vs 0.683) and sensitivity(71.4% vs 47.6%) of hsa-miR-23a-3p were higher than CEA. Conclusion] The hsa-miR-23a-3p is highly expressed in patients with lung adenocarcinoma and is associated with lymph node metastasis,tumor size and TNM staging,indicating that it might be used the diagnosis of lung adenocarcinoma.