| HPLC-DAD波长切换法同时测定身痛逐瘀汤物质基准中8种有效成分含量 |
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| 引用本文： | 成颜芬,王升菊,吴亿晗,王潇,王婷,裴瑾,章津铭,傅超美. HPLC-DAD波长切换法同时测定身痛逐瘀汤物质基准中8种有效成分含量[J]. 中国实验方剂学杂志, 2020, 26(4): 16-22 |
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| 作者姓名： | 成颜芬 王升菊 吴亿晗 王潇 王婷 裴瑾 章津铭 傅超美 |
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| 作者单位： | 成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院, 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地, 成都 611137 |
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| 基金项目： | 中国科协第四届青年人才托举工程项目（2018QNRC1-01）;四川省"千人计划"青年人才项目;成都中医药大学"杏林学者"学科拔尖人才科研提升计划项目 |
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| 摘 要： | 目的:采用高效液相色谱-二极管阵列检测器(HPLC-DAD)波长切换法同时测定15批身痛逐瘀汤物质基准中肌苷,马钱苷酸,绿原酸,苦杏仁苷,羟基红花黄色素A,龙胆苦苷,阿魏酸,甘草苷8种有效成分的含量。方法:采用Thermo Hypersil GOLD C18色谱柱(4. 6 mm×250 mm,5μm),以乙腈-0. 1%磷酸水溶液为流动相梯度洗脱,检测波长分别为248 nm(0～11 min,检测肌苷),235 nm(11～14 min,检测马钱苷酸),324 nm(14～16 min,检测绿原酸),220 nm(16～19 min,检测苦杏仁苷、羟基红花黄色素A),274 nm(19～26 min,检测龙胆苦苷),247 nm(26～54 min,检测阿魏酸、甘草苷),流速设定1. 0 m L·min-1,柱温25℃。根据样品中8个成分的含量,采用SIMCA 14. 1软件对15批身痛逐瘀汤物质基准样品进行正交偏最小二乘法-判别分析(OPLS-DA),以评价各批样品之间的质量差异。结果:各待测组分分离度良好,肌苷,马钱苷酸,绿原酸,苦杏仁苷,羟基红花黄色素A,龙胆苦苷,阿魏酸,甘草苷的质量浓度分别在2. 1～67. 2,1. 812 5～58,1. 937 5～62,5. 212 5～166. 8,8. 45～270. 4,7. 075～226. 4,1. 775～56. 8,3. 875～124 mg·L-1(r均≥0. 999 6)与峰面积呈良好线性关系;平均加样回收率分别为99. 23%,100. 09%,99. 33%,98. 85%,99. 15%,98. 75%,99. 42%,98. 96%; RSD均2%。15批供试品中上述8种成分的质量分数依次为0. 183 5～0. 250 3,0. 173 1～0. 265 3,0. 069 5～0. 169 8,0. 959 2～1. 458 2,1. 905 4～2. 553 3,0. 933 3～1. 997 5,0. 084 6～0. 143 4,0. 212 5～0. 704 3 mg·g-1。通过OPLS-DA发现甘草苷、阿魏酸、龙胆苦苷、羟基红花黄色素A是影响不同批次身痛逐瘀汤物质基准质量贡献较大的4种成分。结论:建立的多成分含量测定的方法结果可靠、操作简便且符合方法学验证要求,适用于身痛逐瘀汤复方制剂研发的质量控制。
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| 关 键 词： | 身痛逐瘀汤 经典名方 波长切换法 有效成分 羟基红花黄色素A 甘草苷 正交偏最小二乘法-判别分析 |
| 收稿时间： | 2019-05-12 |
Simultaneous Determination of Eight Active Components in Material Benchmark of Shentong Zhuyutang by HPLC-DAD Wavelength Switching |
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| CHENG Yan-fen,WANG Sheng-ju,WU Yi-han,WANG Xiao,WANG Ting,PEI Jin,ZHANG Jin-ming and FU Chao-mei. Simultaneous Determination of Eight Active Components in Material Benchmark of Shentong Zhuyutang by HPLC-DAD Wavelength Switching[J]. China Journal of Experimental Traditional Medical Formulae, 2020, 26(4): 16-22 |
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| Authors: | CHENG Yan-fen WANG Sheng-ju WU Yi-han WANG Xiao WANG Ting PEI Jin ZHANG Jin-ming FU Chao-mei |
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| Affiliation: | College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China and College Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China |
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| Abstract: | |
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| Keywords: | Shentong Zhuyutang classical famous formulas wavelength switching method active ingredients hydroxysafflor yellow A liquiritin orthogonal partial least squares discriminant analysis |
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