辽宁省流行的人类免疫缺陷病毒1基因亚型调查

目的：了解辽宁省人类免疫缺陷病毒（human immunodeficiency virus-1,HIV-1)各亚型的流行情况，方法：采集1997年6月至2000年12月间辽宁省检出的16例HIV-1感染者的全血标本，提取核酸后用巢式－聚合酶链反应（nested-PCR)技术扩增HIV-1env基因区，用异源双链泳 动分析法（heteroduplex mobility assay,HMA进行HIV-1亚型的分析，结果：辽宁省目前已有HIV-1A、B'、C和E亚型流行。经性途径感染占全部HIV感染的31．25％，分别为泰国B（B'）亚型和A亚型，以B'为主；经注射吸毒感染占全部HIV感染31．25％，分别为C亚型和E亚型，以C亚型为主，其中1名吸毒者为B或E亚型；经输供血途径感染占全部HIV感染的31．25％，主要为泰国B'亚型，也可见C亚型，垂直传播1名为A亚型。结论：辽宁省已有多种亚型HIV-1毒株流行，有效地控制艾滋病经性乱，吸毒和输供血途径传播的问题已迫在眉睫。

Genetic subtyping of HIV-1 in Liaoning province of China

Objective To study the prevalence of HIV 1 in Liaoning province of China. Methods Nuclear acids were extracted from blood samples of 16 HIV 1 infected individuals collected locally in Liaoning province of China from Jun. 1997 to Dec.2000. The 0.7 kb or 1.2 kb segments of HIV 1 env gene were amplified using nested PCR and the HIV 1 genetic subtypes were then assayed by heteroduplex mobility assay. Results Fifteen of 16 samples were positive by PCR amplification of HIV 1 env region and samples were found to be genetic subtype A,B',C,E. The proportion due to sexual transmission in all HIV infection was 31.25 % (5/15), among which subtype B'(3/5) was the majority. A man who returned from Africa together with his spouse both had type A (2/5) infection. Intravenous drug users (IDUs) took up 31.25 %(5/15) of all the HIV infections. Subtype C (2/4) and E were predominant among intravenous drug users. However, there was one IDU with subtype B or E. Nearly all blood recipients and blood donors were B'(4/5) except one with C. Conclusion There have been several subtypes of HIV 1 existed in Liaoning province, demonstrating the complexity of HIV epidemology in Liaoning province and the difficulty conducting prevention and treatment.