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| 血清铁蛋白时间分辨荧光免疫分析试剂盒的研制 | |
| 引用本文： | 杭建峰,吴英松,徐伟文,周志聪,黄颖,李明. 血清铁蛋白时间分辨荧光免疫分析试剂盒的研制[J]. 免疫学杂志, 2006, 22(Z1): 160-163 |
| 作者姓名： | 杭建峰 吴英松 徐伟文 周志聪 黄颖 李明 |
| 作者单位： | 1. 南方医科大学生物技术学院热带病研究室,广州,510515 2. 达瑞抗体工程技术有限公司质检部,广州,510663 3. 中国药品生物制品检定所药理室,北京,100050 |
| 基金项目： | 广东省广州市科技攻关项目 |
| 摘 要： | 目的 血清铁蛋白(sFER)时间分辨荧光免疫(TRFIA)分析试剂盒的研制.方法 采用双抗体夹心法建立sFER-TRFIA试剂盒,并对试剂盒的各项指标进行评价.结果 试剂盒的线性测量范围为2.2～1 070 ng/mL,灵敏度为0.22 ng/mL,批内、批间的精密度分别为4.6%～7.2%、5.4%～8.9%,与癌胚抗原(CEA)、甲胎蛋白(AFP)和人白蛋白无交叉反应.86份血清样本用本试剂盒与国外其他试剂盒同时检测,其相关系数为0.994.结论 试剂盒各项指标均达到临床检测要求,可替代国外同类产品试剂盒. |
| 关 键 词： | 铁蛋白 时间分辨荧光免疫分析 试剂盒 |
| 文章编号： | 1000-8861(2006)03(S)-0160-04 |
| 修稿时间： | 2005-11-21 |
Preparation of time-resolved fluoroimmunoassay kit for detection of human sermn ferritin |
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| HANG Jian-feng,WU Ying-song,XU Wei-wen,ZHOU Zhi-chong,HUANG Ying,LI Ming. Preparation of time-resolved fluoroimmunoassay kit for detection of human sermn ferritin[J]. Immunological Journal, 2006, 22(Z1): 160-163 | |
| Authors: | HANG Jian-feng WU Ying-song XU Wei-wen ZHOU Zhi-chong HUANG Ying LI Ming |
| Abstract: | Objective To prepare a time-resolved fluoroimmunoassay(TRFIA) kit for detection of human serum ferritin(sFER).Methods The sandwich TRFIA kit for detection of sFER was developed using monoclonal antibody of anti-FER.Results The measure range of kit was 2.2-1 070 ng/mL.The analytical sensitivity was 0.22 ng/mL.There was no cross-reaction with CEA,AFP,and human Albumin.The intra-and inter-assay coefficients of variation were 4.6%-7.2% and 5.4%-8.9%,respectively.The correlation coefficient of the detection results between prepared TRFIA and commercially available FER-TRFIA kit (Wallac) was 0.994.Conclusion sFER-TRFIA kit was a valuable test for clinical application with better sensitivity,specificity,and accuracy. |
| Keywords: | Ferritin Time-resolved fluoroimmunoassay Kit |
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