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重氮乳凝法与胶体金法、聚合酶链反应诊断结核病的比较
引用本文:曾年华,王志斌,李兴国,王珊珊,肖红. 重氮乳凝法与胶体金法、聚合酶链反应诊断结核病的比较[J]. 热带医学杂志, 2002, 2(1): 41-42,45
作者姓名:曾年华  王志斌  李兴国  王珊珊  肖红
作者单位:广州军区军事医学研究所,广州,510507
摘    要:目的 评价应用重氮乳凝试验诊断结核病的临床实用价值。方法 以胶体金法和聚合酶链反应(PCR)为对照,分别用重氮乳凝法和胶体金法检测91份临床诊断为结核病的患者血清标本,以PCR法检测患者相应的痰标本。结果 重氮乳凝法与胶体金法和PCR比较,阳性符合率分别为98.1%和89.8%;敏感性分别为96.3%和72.9%;特异性分别为97.3%和68.8%。结论 重氮乳凝法可用于结核病实验室诊断,尤其适用于现场血清学初筛和基层单位使用。

关 键 词:胶体金法 聚合酶链反应 重氮乳凝法 诊断 结核病
文章编号:0254-900X(2002)01-0041-02

Comparism of the diazopolystyrene latex agglutination test with colloidal gold-technique and PCR for diagnosis of tuberculosis
Zeng Nianhua,Wang Zhibin,Li Xingguo,Wang Sansan,Xiao Hong Medical institute of Guangzhou Military Command,Guangzhou. Comparism of the diazopolystyrene latex agglutination test with colloidal gold-technique and PCR for diagnosis of tuberculosis[J]. Journal Of Tropical Medicine, 2002, 2(1): 41-42,45
Authors:Zeng Nianhua  Wang Zhibin  Li Xingguo  Wang Sansan  Xiao Hong Medical institute of Guangzhou Military Command  Guangzhou
Affiliation:Zeng Nianhua,Wang Zhibin,Li Xingguo,Wang Sansan,Xiao Hong Medical institute of Guangzhou Military Command,Guangzhou 510507
Abstract:Objective To evaluate the applicability of diazopolystyrene latex agglutination test (DLAT) in tuberculosis. Methods With colloidal gold technique and PCR test as control, DLAT was used to detect 91 patients with pulmonary tuberculosis. Results Compared with colloidal gold technique and PCR test and DLAT had positive coincidence rate of 98.1% and 89.8%, sensitives of 96.3% and 72.9% and specificities of 97.3% and 68.8%, respectively. Conclusion DLAT could be used for laboratory diagnosis of TB in China.
Keywords:diazopolystyrene latex agglutination test  diagnosis  tuberculosis
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