原发性肝细胞癌HBx基因缺失突变及意义

Deleted mutation and significance of hepatitis B virus X gene in primary hepatocellular carcinoma

Hepatitis B virus core (HBc) and X (HBx) genes were detected in 45 cases of primary hepatocellular carcinoma (PHC) and their surrounding tissues by polymerase chain reaction amplification. Results indicated that 844% showed HBc gene fragment and among that wild type was 73.7% and 81.6%, mutant type was 263% and 184% in PHC and surrounding tissues, respectively. When HBc and HBx were amplified together, 71.1% and 68.9% were positive in PHC and surrounding tissues, wild type was only 25.0% and 29.0%, and mutant type was 75.0% and 71-0%, respectively. Results revealed that deleted mutation of HBx was frequent compared with HBc alone ( P<0.01). It suggested that deleted mutation of HBx gene may bring a cellular or virus gene close to the enhancer and act as a transactivator effectively, it is very important in hepatocarcinogenesis and replication of hepatitis B virus.