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| 葡萄糖漂移对血管内皮细胞生存的影响 | |
| 引用本文： | 陈真,陈永宁,赵丽,刘国良. 葡萄糖漂移对血管内皮细胞生存的影响[J]. 中国血液流变学杂志, 2007, 17(2): 207-209 |
| 作者姓名： | 陈真 陈永宁 赵丽 刘国良 |
| 作者单位： | 1. 中国医科大学第一临床学院内分泌科,辽宁沈阳,110001 2. 沈阳市铁西区结核病研究所 3. 沈阳市第七医院 |
| 摘 要： | 该研究应用培养人脐静脉内皮细胞株ECV304在5,20,及5/20mmol/L葡萄糖浓度条件下,通过MTT,AnnxenV-FITC检测细胞生存抑制率及凋亡率观察,探讨葡萄糖漂移对血管内皮细胞的作用。结果显示恒定性高葡萄糖细胞生存率低于正常浓度,而波动葡萄糖浓度的生存率较恒定高浓度葡萄糖更低。结论:血糖漂移对血管内皮细胞的损害较恒定性高血糖更严重。是独立于恒定性高血糖之外的血管内皮细胞伤害因素。 |
| 关 键 词： | 凋亡 内皮细胞 葡萄糖漂移 糖尿病 |
| 文章编号： | 1009-881X（2007）02-0207-03 |
| 修稿时间： | 2006-12-04 |
Intermittent High Glucose Enhances Apoptosis in ECV304 in Culture |
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| CHEN Zhen,CHEN Yong-ning,ZHAO Li,LIU Guo-liang. Intermittent High Glucose Enhances Apoptosis in ECV304 in Culture[J]. Chinese Journal of Hemorheology, 2007, 17(2): 207-209 | |
| Authors: | CHEN Zhen CHEN Yong-ning ZHAO Li LIU Guo-liang |
| Affiliation: | Department of Endocrinology, the first affiliated hospital of China Medical University, Shenyang 110001 |
| Abstract: | Objective To explore the effect of fluctuating glucose on ECV304.Method Its cells were incubated for 14 days in media containing different concentrations;5mmol/L,20mmol/L,or adaily alternating 5 or 20 mmol/L glucose.Apoptosis was studied by different methods;viability assay,MTT colorimetric assay,acridine or-ange and flow cytometry assay.Results Intermittent high glucose induces ECV304 apoptosis,furthermore the levels of apoptosis cells enhance more than stable high glucose.Conclusion Variability in glycemic control could be more deleterious to endothelial cells than a constant high concentration of glucose. |
| Keywords: | Apoptosis Endothelial cell fluctuating glucose Diabetes complications |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |