EGF＋61基因多态性与结直肠癌的相关性研究

目的探讨表皮生长因子（EGF）G61A位点（EGF＋61）多态性与结直肠癌发生、发展的关系。方法应用多聚酶链式反应一限制性片段长度多态性（PCR—RFLP）技术，检测中国人群中174例结直肠癌患者和227例正常对照者中EGF＋61位点的基因型，分析其基因型及等位基因的分布频率。结果EGF＋61位点AA基因型及A等位基因在对照者中的分布频率均高于结直肠癌患者（7．0％VS5．7％，30．4％vs29．0％），但其差异均无统计学意义（P〉0．05）。EGF＋61位点AA基因型及A等位基因在不同临床病理分期结直肠癌中的分布频率及规律不一，但其差异均无统计学意义（P〉0．05）。结论在中国人群中，EGF＋61位点的基因多态性与结直肠癌的发生和发展无密切相关性。

Relationship between Polymorphism of Epidermal Growth Factor5'UTR Variant G61A Gene and Colorectal Cancer in Patients in China

Objective To explore the relationship between epidermal growth factor （EGF） ＋61 polymorphism and the occurrence as well as progression of coloreetal cancer （CRC） in Chinese patients. Methods EGF ＋ 61 polymorphism was analyzed among 174 CRC patients and 227 healthy controls by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） to evaluate for the distribution frequency of the genotype and allele. Results The distribution frequency of AA genotype and A allele frequency of EGF ＋ 61 polymorphism were higher in healthy controls than CRC patients （7.0% vs 5.7% , 30.40/0 vs 29.0% ） ,but no significant difference was observed in the distribution （P 〉 0.05 ）. And the distribution frequency of AA genotype and A allele frequency of EGF ＋ 61 polymorphism were different in all kinds of clinicopathological stages of CRC, but there was still no significant difference in the distribution （ P 〉 0.05 ）. Conclusion The polymorphism of EGF ＋ 61 gene showed no close relationship to the occurrence and progression of CRC in Chinese patients.