Survivin蛋白在喉鳞状细胞癌中的表达及意义

目的:探讨Survivin蛋白在喉鳞状细胞癌(LSCC)组织中的表达及其相关性。方法:采用免疫组织化学SP法检测64例LSCC、28例正常喉黏膜及20例喉良性病变组织中的Survivin、Bcl2蛋白的表达,结合临床病理特征进行统计分析。结果:64例LSCC组织中,Survivin蛋白表达阳性43例;28例正常喉黏膜和20例喉良性病变中分别表达1例和2例。Survivin蛋白在声门上型LSCC中表达32例,声门型中表达11例;中低分化的标本中表达34例,高分化的标本中表达9例;在临床分期的Ⅲ、Ⅳ期标本中表达33例,在Ⅰ、Ⅱ期标本中表达10例。以上各项指标之间差异具有统计学意义(均P<0.05)。在有淋巴结转移的标本中表达30例,无淋巴结转移的标本中表达13例,两者之间差异具有统计学意义(P<0.01)。在LSCC组织中Survivin与Bcl2蛋白表达有明显相关性(P<0.05)。结论:Survivin过度表达提示LSCC临床分期晚、分化程度低、易发生淋巴结转移,对于LSCC的早期诊断和预防具有重要参考意义。Bcl2与Survivin的表达可能在LSCC的发病机制中起协同作用。

Expression of Survivin and Bcl-2 in laryngeal squamous cell carcinoma and their significance

Objective:To research the expression of Survivin and Bcl-2 protein in laryngeal squamous cell carcinoma (LSCC) and the correlation between them.Method:Immunohistochemical (SP) method was used to detect the expression of Survivin and Bcl-2 in 64 cases of LSCC,28 cases of normal laryngeal mucous membrane and 20 cases of laryngeal benign lesion.The relationship among them was studied by statistical analysis.Result:Survivin protein was expressed in 43 of 64(67.2%) samples of LSCC, just 2 samples was expressed in laryngeal benign lesion(10%) and 1 sample expressed in normal laryngeal mucosa(3.6%) . The over expression of Survivin was related to the tumor's location, clinical stage, histological grade, lymphatic metastasis.The expression of Survivin was correlated with the expression of Bcl-2.Conclusion:Survivin may play an important role in the pathway of carcinogenesis, progress and metastasis of LSCC,it is feasible for early diagnosis and precaution. Bcl-2 and Survivin may play synergetic roles in the pathogenesis of LSCC.