玉米须总黄酮抗炎镇痛及对急性痛风性关节炎的改善作用研究

目的 研究玉米须总黄酮抗炎镇痛及对家兔急性痛风关节炎的改善作用。方法 预ig给予小鼠玉米须总黄酮（2.12、1.06、0.53 g/kg）7 d,进行耳肿胀实验、热板实验和扭体实验,观察小鼠耳肿胀度、出现舔足时间、出现扭体时间及扭体次数;预ig给予家兔玉米须总黄酮（0.58、0.29、0.15 g/kg）7 d,右踝关节腔内注射尿酸钠溶液诱导急性痛风性关节炎,制作家兔膝关节软骨组织病理切片,观察病理变化。结果 与对照组比较,玉米须总黄酮中、高剂量组小鼠耳肿胀度显著降低（P<0.05）,出现舔足时间显著延长（P<0.05）,高剂量组出现扭体时间显著延长、扭体次数显著减少（P<0.05）;家兔急性关节炎实验,与模型组比较,玉米须总黄酮组滑膜组织炎症明显减轻,充血、水肿、滑膜增生不明显,少量炎细胞浸润。结论 玉米须总黄酮发挥抗炎镇痛作用,且对家兔急性痛风性关节炎具有改善作用。

Anti-inflammatory, analgesic and analgesic effects of total flavone from Stigma maydis and its effect on acute gouty arthritis

Objective To explore total flavone from Stigma maydis in experimental anti-inflammatory analgesic effect in mice and its effects on rabbit acute gouty arthritis.Methods Mice were ig pretreated with total flavone from S. maydis (2.12, 1.06, and 0.53 g/kg) for 7 d. Ear swelling, hot plate test and twisting test were used to observe the ear swelling, the time before licking feet, the time before twisting and the number of writhing. Rabbit were ig pretreated with total flavone from S. maydis (0.58, 0.29, and 0.15 g/kg) for 7 d. Injection of sodium urate solution in the right ankle joint to induce acute gouty arthritis. Rabbit knee joint cartilage tissue pathological slices were made to observe articular cartilage tissue pathological changes.Results Compared with control group, the ear swelling of total flavone from S. maydis high and medium dose group decreased significantly (P< 0.05), and the time before licking foot was prolonged significantly (P< 0.05). The time before twisting prolonged significantly and the number of twisting body decreased significantly in high-dose group (P< 0.05). In rabbit acute arthritis, compared with model group, synovial tissue inflammation in total flavone from S. maydis group significantly reduced, hyperemia, edema, and synovial hyperplasia were not obvious, a small amount of inflammatory cells infiltration.Conclusion Total flavone from S. maydis has certain anti-inflammatory analgesic effects, which has a certain therapeutic effect on rabbit acute arthritis.