注射用姜黄素脂质体过敏现象的初步研究

目的 比较3种不同粒径注射用姜黄素脂质体（CLI）引起的过敏反应，并判断可能的过敏反应类型。方法 将平均粒径约为70、100、130 nm的CLI （剂量为20 mg/kg，以姜黄素计）分别尾iv给予大鼠，分别于注射前，注射后10 min、7 d，眼眶采血，分离血清，ELISA法检测注射前后血清中过敏相关因子——补体C3a、C3b和C5a，组胺和抗体IgE、IgG、IgM的含量变化。将平均粒径约为100 nm的CLI和抗过敏药地塞米松（预处理2 h，2 mg/kg）联用给予大鼠，观察血清中补体C3a、C5a和组胺的含量变化。结果 给予3种粒径的CLI，血清中补体C3a、C3b、C5a和组胺含量均明显升高，与给药前比较差异具有统计学意义（P<0.01）；血清中抗体IgE、IgG、IgM的含量均无明显变化。预先给予地塞米松，在注射CLI前后C3a和C5a的含量无明显变化，组胺含量极低。结论 3种不同粒径CLI在体内引起的过敏反应一致，且可能是补体激活相关的假性过敏反应。

Preliminary study of allergy phenomenon of curcumin liposmes injection

Objective To compare the allergic reactions caused by curcumin liposmes injection (CLI) with different sizes, and analysis the possible type of the allergic reaction caused by those.Methods Curcumin liposomes injection with an average diameter of about 70, 100, and 130 nm were iv administered to rats in tail, and then the concentration of allergy-related factors including complement C3a, C3b and C5a, histamine and antibody IgE, IgG, IgM in serum originated from orbital blood were detected by ELISA before injection and 10 min and 7 d after the injection. Rats were treated with CLI with an average particle size of 100 nm and an anti-allergy drug dexamethasone (pretreated for 2 h, 2 mg/kg). The contents of complement C3a, C5a and histamine in serum were observed.Results Three sizes of CPI could cause the obvious increase of complement factor activation and histamine release, the difference was statistically significant compared with that before administration (P< 0.01). However, there was no significant change in the contents of IgE, IgG and IgM in serum.. Pretreated with dexamethasone, the content C3a and C5a had no obvious change before and after the injection of CLI, the content of histamine was very low.Conclusion The allergic reactions caused by the three sizes of CPI is consistent with each other, which may be the complement activated related pseudo allergic reactions.