海宁市2008年结核分枝杆菌临床分离株MLVA基因分型研究

目的：初步探讨海宁市结核分枝杆菌的数目可变串联重复序列（VNTR）的分型及分布特征,了解海宁市目前结核分枝杆菌的主要流行株,给结核病防治工作提供分子流行病学依据。方法：采用VNTR分型方法,提取DNA,采用PCR和琼脂糖凝胶电泳技术对结核分枝杆菌15个VNTR位点进行检测,并通过BioNumerics 5.0软件进行DNA指纹图谱多态性分析。结果：95株结核分枝杆菌的15个VNTR位点结果显示明显的多态性,经基因聚类分析,共分为10个基因群,共90个基因型。其中群占2.1%,群占1.1%,群占1.1%,群占5.3%,群占1.1%,群占1.1%,群占69.5%,群占1.1%,群占14.7%,群占3.2%。结论：初步证实海宁市2008年的95株结核分枝杆菌VNTRS存在明显的多态性,至少存在10个VNTR群,主要流行群为群。

Genotyping of MLVA isolated from Mycobacterium tuberculosis clinical strains

Objective To investigate the genotyping and distribution pattern of the variable number tandem repeats（VNTR） of Mycobacterium tuberculosis clinical strains isolated in Haining,to understand the main current and to provide molecular epidemiological evidence for the prevention and treatment of tuberculosis.Methods VNTR typing method was used to extract DNA,PCR and agarose gel electrophoresis used to detect 15 Mycobacterium tuberculosis VNTR loci,and DNA fingerprint polymorphism analyzed by BioNumerics 5.0 software.Results The results of 95 Mycobacterium tuberculosis＇15 VNTR loci showed that there were obvious polymorphisms,through genetic analysis,divided into 10 groups and 90 genotypes.Ⅰgroup accounted for 2.1%,Ⅱ group 1.1%,Ⅲ group 1.1%,Ⅳ group 5.3%,Ⅴ group 1.1%,Ⅵ group 1.1%,Ⅶ group 69.5%,Ⅷ group 1.1%,Ⅸ group 14.7%,and Ⅹ group 3.2%.Conclusion The results showed that there were obvious polymorphisms of VNTRs of 95 M tuberculosis clinical strains preliminarily isolated in Haining in the year 2008.There were at least 10 groups,and the main predominant prevalent group was Ⅶ.