应用芪参益气滴丸治疗心肌病的效应与机制研究

目的：通过构建小鼠阿霉素心肌病模型，观察芪参益气滴丸对小鼠心肌病的治疗作用，并初步探讨其机制。方法：取健康雄性昆明小鼠64只，分为1、阿霉素组（ADR组，16只）：生理盐水1mL／100g／d灌胃，ADR4mg．kg。腹腔注射，每周两次，连续4周。2、阿霉素＋芪参益气滴丸①组（16只）：ADR4mg·kg^-1腹腔注射，每周两次，连续4周，第三周开始予芪参益气滴丸3．5mg／100g，d，灌胃给药，累计4周。3、阿霉素＋芪参益气滴丸0纽（16只）：ADR4mg·kg^-1腹腔注射，每周两次，连续4周，同时子芪参益气滴丸3．5mg／100g，d，灌胃给药。4、对照组（16只）：生理盐水1mL／100g／d灌胃，同时生理盐水lmL·kg^-1腹腔注射，每周两次，累计4周。实验第六周末测定小鼠心功能，制作病理HE切片，用光镜行形态学观察，western法检测小鼠Bcl-2、Bax蛋白的表达。结果：1、ADR组小鼠LVESD、LVEDD增大，LVEF明显降低，芪参益气滴丸给药两组小鼠心功能较ADR组均有改善，组间对比有统计学差异（P〈0．05）。2、病理结果显示ADR组心肌细胞变性水肿、排列紊乱，用药①组及0组病理结果均有不同程度的改善。3、Bcl-2蛋白表达阿霉素组〈用药①组〈用药0组〈对照组，Bax蛋白表达阿霉素组〉用药①组〉用药0组〉对照组，组间对比有统计学意义（P〈0．05）。结论：芪参益气滴丸可有效改善阿霉素心肌病小鼠的心功能，纠正心袁，减．轻心肌桶害作用，且早期用药效果佳，其机制与抑制小鼠心肌细胞凋亡有关。

Effect and mechanism of Qishen Yiqi Pills on adriamycin- induced cardiomyopathy in mice

AIM： To study the effect and probable mechanism of Qishen Yiqi Pills on adriamycin （ADR）-induced cardiomyopathy in mice. METHODS： Sixty-four mice were randomly divided into （1） the ADR group： saline （1 mL/100 g） administered every day by intragavage, ADR （4 mg.kg-1） administered to each mouse by intraperitoneal injection twice a week for four weeks; （2） the ADR ＋ Qishen Yiqi Pills I group： ADR （4 mg.kg-1） administered to each mouse by intraperitoneal injection twice a week for four weeks, and at the beginning of the third week Qishen Yiqi Pills （3.5 mg/100 g） administered by intragavage every day for four weeks; （3） the ADR ＋ Qishen Yiqi Pills II group： ADR （4 mg.kg-l） administered to each mouse by intraperitoneal injection twice a week for four weeks, and at the same time Qishen Yiqi Pills （3.5 mg/100 g） administered by intragavage every day for four weeks; （4） the control group： saline （1 mL/100 g） administered every day by intragavage, saline （1 mL.kg-t） administered to each mouse by intraperitoneal injection twice a week for four weeks. Six weeks later, cardiac function, myocardial pathology, and expression of Bcl-2 and Bax were evaluated. RESULTS： 1. The left ventricular diastolic diameter and the left ventricular systolic diameter were significantly increased （P 〈 0.05） and the left ventricular ejection fraction was significantly decreased （P 〈 0.05） in the ADR group, and the cardiac function of both the ADR ＋ Qishen Yiqi Pills I group and the ADR ＋ Qishen Yiqi Pills I group improved. 2. Myocardial morphologic observation showed that the myocardial fibers were disordered, there was cell edema, and gap widening in the ADR group. The degree of myocar- dial cell injury was reduced in the ADR ＋ Qishen Yiqi Pills I group and ADR ＋ Qishen Yiqi Pills II group compared with the ADR group. 3. The expression of Bax in the ADR group was significantly up-regulated, and the expression of Bcl-2 was significantly down- regulated in the ADR group compared with the ADR ＋ Qishen Yiqi Pills I group, the ADR ＋ Qishen Yiqi Pills II group, and the control group （P 〈 0.05）. CONCLUSIONS： Qishen Yiqi Pills can effectively improve the cardiac function of ADR-induced cardiomyopathy, and the earlier it is used is better. The probable mechanism of action may be the inhibition of the apoptosis of myocardial cells.