高效液相质谱联用法分析大鼠血浆中异鼠李素-3．0-新橙皮糖苷的浓度及其药代动力学研究

目的：建立LC-MS／MS方法测定异鼠李素-3-O-新橙皮糖苷的浓度并研究其在大鼠体内的药代动力学。方法：8只大鼠分别尾静脉注射异鼠李素-3-O-新橙皮糖苷5mg·kg^-1。建立并确证高灵敏度的液质联用法分析大鼠血浆中异鼠李素-3-O-新橙皮糖苷的浓度，采用芦丁作为内标。采用甲醇蛋白沉淀法提取血浆中的异鼠李素-3-O-新橙皮糖苷和芦丁。然后用10mmol·L^-1的乙酸铵／甲醇（20：80，v／v）的流动相在C18色谱柱（150mm×2．1mm，I．D.，5μm）上进行洗脱并采用多反应检测的方式进行质谱分析。结果：异鼠李素-3-O-新橙皮糖苷的在大鼠血浆中的线性范围为0．01-10μg·mL^-1。其定量下限为0.01μg·mL^-1。结论：该方法可以用于测定大鼠血浆中异鼠李素-3-O-新橙皮糖苷的浓度。

Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies

AIM： To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats. METHODS： Eight rats were given 5 mg·kg^-1 isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin （internal standard） were extracted with methanol followed by a rapid isocratic elution with 10 mmol.L-1 ammonium acetate buffer/methanol （20 ： 80, V/V） on a C18column （150 mm ×2.1mm,I.D.,5μm） and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode. RESULTS： The assays were linear over the concentration range of 0.01-10 μg·mL^-1 for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL^-1. CONCLUSION： The validated method is successfully applied to determine the plasma concentrations of isorhanmetin-3-O-neohesperidosidein in rats.