AB-8树脂精制柴芩两解汤的工艺研究

目的确定AB-8树脂精制柴芩两解汤的最佳工艺。方法采用L9(34)正交设计,以HPLC法测定其中黄芩苷和栀子苷的吸附、解析率为指标,分别考察动态吸附中药液浓度、树脂量、流速对吸附的影响;以及解析液浓度、体积、流速3个因素对解析的影响。结果吸附药液浓度为0.15 g生药/mL,树脂用量为3倍药材量,速度10 mL/min;解析液为75%乙醇,10倍药材量,流速10 mL/mim,黄芩苷及栀子苷的解析率可达95%以上。结论AB-8树脂精制柴芩两解汤工艺简单、稳定、可控。 

A Study into Technique for Preparing "Chaiqin Liangjie Decoction" with AB-8 Resin

OBJECTIVE To determine the optimal technique for preparing Chaiqin Liangjie Decoction with AB-8 resin.METHOD L_9(3~4) orthogonal method and HPLC were employed to determine the absorption and resolution rates of baicalin and jasminoidin.Using these as the indexes,the author carried out a study into the effect of drug concentration,resin amount and flow rate on absorption,and the effect of resolution solution concentration,volume and flow rate on resolution.RESULT The resolution rate of baicalin and jasminoidin could reach over 95% under the conditions of an absorption solution concentration of 0.15 grams of crude drug per milliliter,a resin amount of 3 times that of the drugs,a flow rate of 10 mL/min;and a resolution solution of 75% alcohol,a resin amount of 10 times that of the drugs and a flow rate of 10 mL/min.CONCLUSION The technique for preparing Chaiqin Liangjie Decoction with AB-8 is simple,stable and controllable.