番茄红素抑制原代前列腺上皮细胞增殖和雄激素受体基因片段活性

目的 观察类胡萝卜素族中番茄红素对原代前列腺上皮细胞增殖和雄激素受体基因片段活性的影响.方法 分离培养获得原代前列腺上皮细胞后,加入1、5、10μmol/L番茄红素溶液,免疫荧光法测定其对原代上皮细胞增殖的影响.将含有构建的雄激素受体基因片段和荧光素酶报告基因的质粒DNA导入培养中的原代细胞,转染后加入1、5、10μmol/L番茄红素溶液,通过荧光光度计的结果评价番茄红素对雄激素受体基因活性的影响.结果与细胞培养基对照组比较,加入1、5、10μmol/L番茄红素溶液后细胞增殖分别减少了3.04%、6.88%和18.42%(P<0.01).与作为番茄红素溶剂的1%四氢呋喃溶剂(THF)对照组比较,加入5、10μmol/L番茄红素溶液后细胞增殖分别减少了3.86%和15.40%(P<0.05).将上述不同浓度番茄红素溶液加入转染后的含有构建的雄激素受体基因片段和荧光素酶报告基因的质粒DNA的原代细胞后,加入1、5、10μmol/L番茄红素溶液后荧光光度计数值分别降低了52.38%、68.10%和95.24%.结论 番茄红素对原代前列腺上皮细胞的增殖有显著抑制作用,它对雄激素受体基因片段的活性也有着抑制作用,并且与剂量呈正相关.

Lycopene inhibiting cell proliferation and androgen receptor gene element activity in primary prostate epithelial cells

Objective To evaluate the impact of lycopene on cell proliferation and androgen receptor gene activity of primary prostate epithelial cells.Methods Lycopene at concentrations of 1,5,10 μmol/L was added to the isolated primary prostate epithelial cells and the impact on cell proliferation was analyzed by using immunofluorescent method.A transient transfection of a plasmid DNA recombinant containing androgene receptor element-luciferase (ARE-Luc) report gene into primary prostate epithelial cells was developed and the impact of different concentrations of lycopene on androgen receptor element was evaluated by using quantative analysis of luciferse enzyme product.Results Lycopene ( 1,5,10μmol/L)could inhibit cell growth by 3.04%,6.88% and 18.42% ( P ＜ 0.01 ) respectively as compared with PrEBM media.As compared with 1% THF solvent,the proliferation in lycopene-treated groups (5,10μmol/L) was inhibited by 3.86% and 15.40% ( P ＜0.05 ) respectively.The results of transient transfection and luminometer data showed that luminometer data were decreased by 52.38%,68.10% and 95.24% respectively when 1,5,10 μmol/L lycopene was added.Conclusion Lycopene inhibits cell proliferation and androgen receptor gene element activity in primary prostate epithelial cells in a dose-dependent manner.