| 小蔓长春花中长春胺含量测定方法的研究 |
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| 引用本文： | 何丽一,周金云,方起程. 小蔓长春花中长春胺含量测定方法的研究[J]. 药学学报, 1983, 18(12): 925-928 |
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| 作者姓名： | 何丽一 周金云 方起程 |
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| 作者单位： | 中国医学科学院药物研究所,北京 |
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| 摘 要： | 本文报道测定小蔓长春花中长春胺含量的两个方法——可见薄层扫描法及紫外分光光度法。前法的线性范围为0.5～3.0μg/点,纯品的变异系数为2.02%(n=9);后法在50～250μg/IN HCl5ml间符合Beer定律,纯品回收率为98.2±2.1%。两法测定结果一致,但前法较快速,灵敏。
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| 关 键 词： | 小蔓长春花 长春胺 薄层层析 扫描法 分光光度法 |
| 收稿时间： | 1982-08-03 |
STUDIES ON DETERMINATION OF VINCAMINE IN VINCA MINOR |
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| HE Li-yi,ZHOU Jin-yun and FANG Qi-cheng. STUDIES ON DETERMINATION OF VINCAMINE IN VINCA MINOR[J]. Acta pharmaceutica Sinica, 1983, 18(12): 925-928 |
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| Authors: | HE Li-yi ZHOU Jin-yun FANG Qi-cheng |
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| Abstract: | Application of thin layer chromatographic method to separation and subsequent visible densitometric or UV spectrophotometric determination of vincamine in Vinca minor is described.By densitometric method the linear range was 0.5～3.0μg, per spot and CV% of pure vincamine was 2.02 (n=9).Beer's law was obeyed in the range of 50～250μg in 5 ml of 1 N hydrochloric acid solution and recovery of pure vincamine was 98.2±2.1% by the UV spectrophotometric method.The results of quantitative determination of 6 samples by both methods agreed very well, but. the densitometric method was far more rapid and sensitive than the UV method. |
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| Keywords: | Vincamine Thin layer chromatography Densitometric method Spectrophotometric method Vinca minor |
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