| 3,5-diBr-DMPAP光度法测定全血铁 |
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| 引用本文： | 杨光煜,叶小琴,蔡文选.3,5-diBr-DMPAP光度法测定全血铁[J].微量元素与健康研究,2002,19(2):59-60. |
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| 作者姓名： | 杨光煜 叶小琴 蔡文选 |
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| 作者单位： | 浙江省温州医学院化学教研室,温州,325027 |
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| 摘 要： | 目的 :建立一种快速、简便、灵敏分光光度法测定全血中微量铁。方法 :在表面活性剂 Tri-ton X-1 0 0存在下 ,用 2 -(3 ,5 -二溴 -2 -吡啶偶氮 ) -5 -二甲氨基酚 (简称 3 ,5 -di Br-DMPAP)作显色剂直接光度法测定全血中微量铁。结果 :该方法在 p H4.0的乙酸 -乙酸钠缓冲液中 ,以 746nm为测定波长 ,可选择性测定全血中微量铁 ,本法线性范围为 0～ 1 0μg/ 3 .4ml,表观摩尔吸光数为 2 .75× 1 0 4 L/ (mol· cm) ,回收率为 98.4%～ 1 0 1 .3 %。结论 :该法具有操作快速、简便、结果灵敏可靠等优点 ,适合临床应用
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| 关 键 词： | 全血 铁 3 5-diBr-DMPAP 分光光度法 |
| 文章编号： | 1005-5320(2002)02-0059-02 |
| 修稿时间： | 2002年12月11 |
A new spectrophotometric method to determing iron in human blood With 3,5—diBr—DMPAP |
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| YANG Guang-Yi,YE Xiao-Qin,CAI Wen-Xuan.A new spectrophotometric method to determing iron in human blood With 3,5—diBr—DMPAP[J].Studies of Trace Elements and Health,2002,19(2):59-60. |
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| Authors: | YANG Guang-Yi YE Xiao-Qin CAI Wen-Xuan |
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| Abstract: | Objective:To extablish a simple sensitive and stable method of determination of iron in human blood by UV-Spectrophotometry.Methods:For determination of iron in human hair with 3,5-diBr-DMPAP in the presence of surfactant Triton X-100.Results:The maximum absorption of the complex compound was at 746 nm,The linearity of test was up to10 μg/3.4 ml,The molar absorption coefficient is 2.75×10 4L/(mol·cm).The recoveries ranged from 98.4%～101.3%.common coexistent substances don's interfere with the determination.Conclusions:This method is simple.sensitive,rapid and accurate,and suitable for clinical application. |
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| Keywords: | human blood iron 3 5-diBr-DMPAP spectrophotometry |
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