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| RP-HPLC法测定黄芩苷脂质体的体外释放度 | |
| 引用本文： | 刘硕,仵文英,席枝侠,王娜,黄洁.RP-HPLC法测定黄芩苷脂质体的体外释放度[J].中国药房,2007,18(33):2594-2596. |
| 作者姓名： | 刘硕 仵文英 席枝侠 王娜 黄洁 |
| 作者单位： | 1. 北京胸科医院药剂科,北京市,101149 2. 西安交通大学医学院第二附属医院药剂科,西安市,710004 3. 西安交通大学医学院药学系,西安市,710061 |
| 摘 要： | 目的:建立以反相高效液相色谱法测定黄芩苷脂质体的体外释放度的方法。方法:色谱柱为岛津C18(150mm×4.6mm,5μm),流动相为甲醇-水-磷酸(47∶53∶0.2),流速为1.0mL.min-1,检测波长为280nm,柱温为25℃,进样量为10μL。结果:黄芩苷检测浓度在1.01～80.80μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为100.05%,RSD=0.93%。结论:本方法灵敏、简便、准确,可用于黄芩苷脂质体的体外释放研究。 |
| 关 键 词： | 反相高效液相色谱法 黄芩苷 脂质体 体外释放度 |
| 文章编号： | 1001-0408（2007）33-2594-03 |
| 收稿时间： | 2006-11-24 |
| 修稿时间： | 2007-04-20 |
Determination of in Vitro Drug Release of Baicalin Liposomes by HPLC |
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| LIU Shuo,WU Wenying,XI Zhixia,WANG Na,HUANG Jie.Determination of in Vitro Drug Release of Baicalin Liposomes by HPLC[J].China Pharmacy,2007,18(33):2594-2596. | |
| Authors: | LIU Shuo WU Wenying XI Zhixia WANG Na HUANG Jie |
| Abstract: | OBJECTIVE:To establish a HPLC method for the determination of in vitro drug release of Baicalin liposomes.METHODS:The separation was performed on a C18 column(150mm×4.6mm,5μm)at 25℃;the mobile phase was composed of methanol-water-phosohoric acid(47∶53∶0.2)at a flow rate of 1.0mL·min-1.The ultraviolet wavelength was set at 280nm and the sample size was 10μL.RESULTS:The calibration curve showed good linearity in the range of 1.01～80.80μg·mL-1(r=0.999 8)and the average recovery rate was 100.05%(RSD=0.93%).CONCLUSION:The method is sensitive,simple and accurate,and applicable for the study of in vitro drug release of Baicalin liposomes. |
| Keywords: | RP-HPLC Baicalin Liposomes In vitro release |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |