阿霉素对肝癌细胞系MHCC97-L中Wnt5b和Nanog表达的影响

目的:探讨干细胞相关基因Wnt5b和Nanog在阿霉素(ADM)作用下的人低转移性肝癌细胞系MH-CC97-L中的表达。方法:MHCC97-L细胞常规培养,用四甲基偶氮唑盐比色法(MTT)检测ADM作用72 h时MHCC97-L的半数致死量(LD50);蛋白质免疫印迹法(Western blot)检测ADM(LD50)作用下,干细胞相关基因Wnt5b和Nanog蛋白表达。结果:MTT实验结果显示,ADM对MHCC97-L的半数致死量(ID50)为0.423 3μmol/L;Western blot检测发现,在ADM(ID50)作用下,随作用时间延长,Wnt5b蛋白表达水平逐渐增高,12 h时达最高峰(P<0.05),以后则逐渐降低,72 h时降到最低(P<0.05);在ADM(ID50)作用下,随作用时间延长,Nanog蛋白表达水平逐渐降低,12 h时降到最低,以后则逐渐升高,72 h时达到最高(P<0.05)。结论:干细胞相关基因Wnt5b、Nanog在ADM作用下的人肝癌细胞系MHCC97-L中均有不同程度的表达。

Effects of Adriamycin on the Expression of Wnt5b and Nanog in Hepatocellular Carcinoma Cell Line MHCC97-L

Objective: To explore the expression of stem cell associated gene Wnt5b and Nanog in hepatocellular carcinoma cell line MHCC97-L incubating with adriamycin(ADM).Methods: MHCC97-L cells were routinely cultured.Median lethal dose(LD50) of MHCC97-L treated with ADM for 72 h were determined with MTT method.The expression of Wnt5b and Nanog proteins in MHCC97-L after treating with ADM(LD50) was detected with Western blot.Results: MTT results showed that LD50 of MHCC97-L treated with ADM was 0.423 3 μmol/L.The expression of Wnt5b increased when MHCC97-L was exposed to ADM within 4 hours,and reached peak at 12 hours,and then decreased gradually,and reduced to minimum at 72 h.When the incubation time was less than 12 h,Nanog decreased gradually with the time passing(P<0.05),and reduced to minimum at 12 h,and then it increased gradually,reached peak at 72 hours.Conclusions: Wnt5b and Nanog proteins express in MHCC97-L exposed to ADM in different degrees.