海洛因戒断、复吸对大鼠肾上腺髓质细胞神经肽Y表达的影响

目的:探讨海洛因戒断、脱毒、复吸对大鼠肾上腺髓质细胞神经肽Y(NPY)表达的影响。方法:成年雄性SD大鼠63只,随机分为实验组、盐水对照组和正常对照组,实验组又分为戒断组、脱毒组和复吸组,采用免疫组织化学及图像分析法,观察3组大鼠肾上腺髓质NPY的表达。结果:光镜下,肾上腺髓质NPY免疫反应(IR)细胞免疫反应产物呈棕黄色细颗粒状,存在于胞质内;与正常对照组及盐水对照组比较,戒断组和复吸组肾上腺髓质NPY-IR细胞免疫染色加深,图像分析平均灰度值低于正常对照组及盐水对照组(P<0.05);脱毒组与正常对照组比较,NPY-IR细胞染色强度、平均灰度值差异无统计学意义(P>0.05)。结论:在海洛因戒断及复吸期间,NPY在肾上腺髓质细胞表达增强,提示肾上腺髓质细胞分泌的NPY增多;脱毒治疗组大鼠NPY表达与正常组NPY差异无显著性,提示脱毒治疗后肾上腺髓质细胞分泌的NPY减少可能与其功能逐步趋于正常有关。

The Effect of Abstinence and Relapse to Heroin on the Expression of Neuropeptide Y in Rat Adrenal Medulla Cells

Objective: To explore the effect of abstinence,detoxication and relapse to heroin on the expression of neuropeptide Y(NPY) in rat adrenal medulla cells.Methods: Sixty-three adult male SD rats were randomly divided into normal control group(group NC),saline control group(group SC)and experiment groups including abstinence group(group A),detoxication group(group D) and relapse group(group R).The expression of NPY in rat adrenal medulla cells was detected with immunohistochemical method and image analysis method.Results: Under light microscope,the brownish yellow granules which were immunoreactive products existed in the cytoplasm of NPY immunoreactive(IR) cells.Compared with those in groups NC and SC,the immunostaining of NPY-IR cells was deeper,and the mean grey degrees were lower obviously in groups A and R(P<0.05);No statistical differences were found in immunostaining strength and mean grey degree of NPY-IR cells between group D and normal control group(P>0.05).Conclusions: During abstinence and relapse to heroin,the expression of NPY in rat adrenal medulla cells increases obviously,which suggests that adrenal medulla cells secrete NPY increasingly;No statistical difference was found in the expression of NPY between group D and normal control group,which suggests that the secretion of NPY in rat adrenal medulla decreases after accepting detoxication treatment,which may relat to the recovery of NPY function.