人胚胎中枢神经系统NOVmRNA神经元的发育--原位杂交研究

目的 研究人胚胎中枢神经系统ＮＯＶ ｍＲＮＡ神经元的发育。方法 地高辛标记的ｃＲＮＡ探针原位杂交技术。结果 （１）脊髓：第１６周人胚胎脊髓腹侧出现ＮＯＶ ｍＲＮＡ神经元，为大型神经元，至第２８周扩展到后角中间神经元，第３８周中央灰质中阳性神经元明显。（２）延髓：第１６周延髓观察到下橄榄核、舌下神经核、迷走神经背核均有阳性神经元分布，至第２８周，三叉神经脊束核和楔束副核检测到阳性神经元；（３）脑桥、

DEVELOPMENT OF NOV mRNA NEURONS IN HUMAN FETAL CENTRAL NERVOUS SYSTEM--IN SITU HYBRIDIZATION STUDY BY cRNA PROBE

Objective\ By using hIL 2 muscle model to exploit a new gene therapeutics for cancer.Methods\ Two mammalian expression vectors were constructed,which contained 1kb human interleukin 2 cDNA,and were transfected into primary skeletal muscle cells of the rats mediated by lipofectin(hIL 2 muscle model).The hIL 2 expression in primary muscle cells was detected by immunohistochemical stain method.Results\ Identification of pCMV IL 2 and PRSV IL 2 was confirmed by digesting with restrictive endonucleases,about 10% positive cells were observed and the expression level of pCMV IL 2 and pRSV IL 2 had no difference in the muscle cells.Conclusion Recombinant plasmid DNAs might be suitable for gene therapeutics for cancer.