survivin反义寡核苷酸转染对人乳腺癌细胞系MCF 7生长及对长春瑞滨的增敏作用

目的探讨survivin反义寡核苷酸（ASODN）对乳腺癌细胞系MCF 7的诱导凋亡、抑制增殖和对长春瑞滨的增敏作用。方法采用脂质体介导survivin ASODN转染乳腺癌细胞系MCF 7，半定量RT PCR方法检测survivin基因mRNA表达，Western blot方法检测survivin基因蛋白表达，AnnexinⅤ/PI双染法通过流式细胞仪检测细胞凋亡率变化，PI染色通过流式细胞仪检测细胞周期时相变化，MTT比色法检测转染细胞的生长抑制情况及对长春瑞滨的敏感性,电镜观察反义转染对乳腺癌细胞系MCF 7的凋亡诱导作用。结果survivin反义复合物下调MCF 7细胞的survivin mRNA和蛋白表达，并呈剂量依赖性。细胞周期被阻滞于G2/M期，细胞凋亡率明显升高（P＜0.05），反义组细胞生长抑制率明显上升（P＜0.05）。透射电镜下转染组细胞呈凋亡晚期变化。600?nmol/L反义转染组长春瑞滨的IC50值为(3.7±0.42)μg/mL,正常组为(7.1±0.68)μg/mL,差异有统计学意义(P＜0.05)。结论survivin反义寡核酸可有效下调MCF 7的survivin mRNA及蛋白表达水平,抑制增殖，诱导凋亡，并增强其对化疗药物长春瑞滨的敏感性。

Effect of survivin antisense oligonucleotide antisense transfection on growth and sensitivity to vinorelbine in the human breast cancer cell MCF-7

Objective To explore the effects of survivin antisense oligonucleotide(ASODN) on apoptosis,growth and sensitivity to vinorelbine in the human breast cancer cell MCF-7.Methods Survivin-ASODN was transfected into the MCF-7 cells mediated by the Lip reagent.Expression of survivin mRNA was determined by RT-PCR and of survivin protein by Western blot.Apoptosis rate and cell cycle changes in antisense oligonucleotide(ASODN) transfected cells were detected by FCM.Cell proliferation and chemo-sensitivity to vinorel...