检测颅脑外伤患者血清S100B蛋白和一氧化氮浓度的临床意义

目的 探讨颅脑外伤患者脑损害程度与血清S100B蛋白和一氧化氮浓度的关系.方法 用双抗体夹心ELISA法和硝酸还原法检测30名健康人和108例颅脑损伤患者血清S100B蛋白和一氧化氮水平(男65例,女43例,年龄为16～65岁;格拉斯哥昏迷分级(GCS)评分为3～5分,平均10.4分.其中重型颅脑外伤38例,中型颅脑外伤患者组36例,轻型颅脑外伤患者组34例.结果 108例颅脑外伤患者在伤后12 h重型、中型和轻型血清S100B水平分别是(1.67±0.21)μg/L、(1.22±0.37)μg/L及(0.84±0.25)μg/L],与对照组(0.38±0.12)μg/L]相比,差异有统计学意义,P<0.01.而重型患者伤后12 h血清S100B蛋白水平比轻、中型患者也明显增高,P<0.01.重型颅脑外伤患者的血清一氧化氮浓度(72±19)μmol/L]均明显高于对照组(50±7)μmol/L],P<0.01;重型颅脑外伤患者与轻型、中型组比较差异有统计学意义(P<0.05);而轻、中型组与对照组比较差异无统计学意义(P>0.05).结论 血清S100B可作为诊断早期脑损伤的指标.

The detection of sera S100B and NO of patients with brain injury

Objective To explore the changes of sera S100B and NO and their significance of patients with brain injury. Method We detected sera S100B and NO level of 30 healthy persons and 108 cases with brain injury by ELISA and method. Results The sera S100B level of patient group was increased after 12 h brain injury,and sera NO concentrations of sereve type were significantly high than control group, P<0.01. NO concentrations of sereve type, middle type and minor type were significantly high than control group, middle type and minor type group. P<0.05, However ,sera NO level of middle type and minor type group of brain injury had no significance than control group, P>0.05. Conclusion Sera S100B examinations can be indicas as diaognosis of early brain injury.