阻断CD18介导的白细胞粘附对岛状皮瓣成活的影响

目的研究白细胞及白细胞粘附在缺血再灌注损伤中的作用。方法应用大鼠腹部岛状皮瓣模型，检测了皮瓣髓过氧化酶（ＭＰＯ）和丙二醛（ＭＤＡ）含量，观察了皮瓣的成活情况。结果与正常时相比，缺血８小时及再灌注１小时皮瓣ＭＰＯ和ＭＤＡ水平明显增高，而以抗ＣＤ１８单抗阻断白细胞粘附可明显减轻这种变化，并有效地提高皮瓣的存活面积。结论ＣＤ１８介导的白细胞粘附参与了皮瓣再灌注损伤过程，抗ＣＤ１８单抗阻断白细胞粘附能减轻白细胞介导的组织损伤，并对岛状皮瓣具有保护作用。

Effects of blocking CD 18 mediated leukocyte adhesion on the survival of the island flap

Objective This study was to investigate the role of leukocyte and leukocyte adhesion in tissue injury from ischemia and reperfusion.Methods The experiment utilized the monoclonal antibody (mAb) directed to the leukocyte adhesion glycoprotein CD 18 to block leukocyte adhesion and aggregation in an island flap model in rats.Tissue content of myeloperoxidase (MPO) and malondialdehyde (MDA) were detected after transient treatment with either saline or mAb directed to CD 18 .Flap survival was assessed 7 days afterwards.Results The content of both MPO and MDA was significantly increased with 8h ischemia and 1h reperfusion of the flap.The treatment with anti CD 18 mAb significantly decreased the levels of MPO and MDA and also significantly improved the survival of flaps compared with the saline treated controls.Conclusion CD 18 mediated leukocyte adhesion plays an important role in tissue injury from ischemia and reperfusion.Blocking leukocyte adhesion can attenuate leukocyte mediated injury,providing protective effects on island flaps.