恶性肿瘤染色体端粒行为异常与癌变发生的关系研究

 目的　探讨恶性肿瘤染色体端粒行为异常与癌变发生的关系。方法　粒端联合采用微量全血培养法制备淋巴细胞染色体 ;端粒长度用 Southern杂交法 ;端粒酶活性用 TRAP- PCR法。结果　食管癌、鼻咽癌、肺癌染色体端粒联合率分别为 1 4.38%、35.0 7%、2 4 .40 % ,均高于对照组9.83%、2 0 .2 7%、1 5.60 % (P<0 .0 1 ) ;鼻咽癌、肺癌端粒长度变化分别为伸长 1 6.67%、1 6.67% ,缩短 76.67%、69.0 4 % ,长度无变化 6.66%、1 4.2 9% ;端粒酶活性阳性率为鼻咽癌 82 .1 4% ,肺癌 (非小细胞肺癌 NSCLC 78.3% ,小细胞肺癌 SCLC 1 0 0 % )。结论　端粒联合率的增高、端粒长度的缩短及端粒酶活性表达与细胞癌变密切相关。

Study on the Relationship between the Behavioral Abnormality of Chromosomal Telomeres in Malignant Neoplasm's and the Carcinogenesis

Objective Exploration of the relationship between the behavioralabnormality of chromosomal telomeres in malignant neoplasm's and the carcinogenesis.Methods The telomeric association was used by peripheral blood lymphocyte cultures,the telomere length was used by means of southern hybridization,the telomerase activity was used by means of TRAP(telomeric Repeat Amplification protocol)on PCR.Results The telomeric association rate of esophageal carcinoma (14.38%),Nasopharyngeal Carcinoma NPC (35.07%),Lung Carcinoma(24.40%) were found to be higher than that of controls (9.83%、20.27% and14.29%)P<0.01,the telomere length of in NPC and lung carcinoma 16.67% and 16.67% have telomere elongation,76.67% and 69.04%have telomere reduction,6.67% and 14.29% exhibited equal lengths as compared with the controls.The positive rates of telomerase activity were 82.14% in NPC,78.3% in NSCLC、100% in SCLC.Condusion Increase of the telomeric association retes,the reduction of telomere length and the positive rates of telomerase activity were more closely related to cell carcinogenesis.