氟对游离毛囊凋亡影响的实验研究

目的观察离体培养条件下氟对人头皮毛囊各部位凋亡的影响及硒对氟影响的拮抗作用。方法构建人头皮游离毛囊培养模型,在模型中,加入不同浓度的氟化钠和亚硒酸钠,筛选浓度后,分为不同组;制作冰冻切片,利用TUNEL法原位检测各组毛囊不同位置的凋亡数量并做统计分析;用透射电镜进行观察照相。结果原位凋亡染色发现,1mmol/L和10mmol/L的氟化钠能使游离培养5d的人头皮毛囊中外根鞘、真皮鞘和毛乳头,及毛球下部的凋亡细胞显著增多,0.01mmol/L的亚硒酸钠能拮抗1mmol/L氟化钠对外根鞘、毛球部的促凋亡作用(P<0.05)。结论不同浓度的氟化钠对游离培养的第5天的人头皮毛囊的凋亡具有不同作用,一定浓度的氟化钠能促进游离毛囊一定部位的细胞凋亡。0.01mmol/L的亚硒酸钠对一定浓度的氟化钠的促凋亡作用具有拮抗性。

Apoptotic study on the effect of fluorine and selenium on the human hair follicle in vitro

OBJECTIVE: The aim of this study was to observe the human hair follical apoptosis status affected by fluorine and the antagonism effect by selenium in vitro. METHODS: The single hair follicles were separated and cultured, then they were added in different concentrations of sodium fluoride and sodium selenite. Chosen the appropriate concentrations, they were divided into 7 groups. The TUNEL was used to investigate the apoptotic cells of different parts. The morphous of hair follicles was observed consecutively and electron microscope was used. RESULTS: We found that in 1 mmol/L and 10 mmol/L sodium fluoride groups, when the human hair follicles in vitro were cultured on the 5th day, the apoptotic cells of outer root sheath (ORS), dermal sheath and hair papilla, hair bulb were obviously increased. But 0.01 mmol/L sodium selenite weakened the toxicity of 1 mmol/L sodium fluoride at the outer root sheath and hair bulb (P < 0.05). CONCLUSIONS: Different concentrations of sodium fluoride had different effect on the growth of human hair follicle in vitro which were cultured on 5th day. Sodium fluoride of certain concentration could accelerate the apoptosis of human hair follicle in vitro. Sodium selenite of certain concentration could act antagonism to the toxicity of sodium fluoride.