非小细胞肺癌组织中表皮生长因子受体与磷酸化Akt蛋白的表达及其意义

目的 评价非小细胞肺癌(NSCLC)标本中表皮生长因子受体(EGFR)与其信号传导通路下游的磷酸化Akt(p-Akt)蛋白质表达的意义.方法 制作189例非小细胞肺癌标本的组织微阵列,运用免疫组织化学方法检测肺癌组织中的EGFR和p-Akt蛋白质的表达,分析该两种蛋白表达与患者预后生存之间的关系.结果 EGFR在NSCLC中的阳性表达率为54.5%(103/189),单因素分析提示其表达程度与性别(x2=4.267,P=0.039),病理类型(x2=14.749,P=0.002),分化程度(x2=6.295,P=0.043)以及病理分期(x2=9.318,P=0.025)相关,而Logistic多因素回归分析显示EGFR表达仅与病理类型之间的相关性具有统计学意义(P=0.047).p-Akt在NSCLC中的阳性表达率为51.3%(97/189),其阳性表达率与各临床病理特征无关(P＞0.05).二者阳性表达均与预后无关(P值分别为0.972和0.903).结论 NSCLC中EGFR和p-Akt蛋白质阳性表达率对患者预后无提示意义,尚不能以EGFR或p-Akt蛋白质表达作为NSCLC分子分期或判断预后的指标.

Epidermal growth factor receptor and phosphorylated Akt protein expression in non-small-cell lung cancer

OBJECTIVE: To evaluate the expressions of epidermal growth factor receptor (EGFR) and phosphorylated Akt (p-Akt) in non-small-cell lung cancer (NSCLC). METHODS: 189 NSCLC samples were made into tissue microarray and immunohistochemistry was used to detect the expression of EGFR and p-Akt proteins. The association of the expressions of EGFR and p-Akt protein with the survival time was evaluated. RESULTS: The EGFR and p-Akt protein positive rates were 54.5% and 51.3% respectively. Univariate analysis showed that the EGFR expression was associated with gender (chi2 = 4.267, P = 0.039), histology (chi2 = 14.749, P = 0.002), differentiation (chi2 = 6.295, P = 0.043), and pathological stage (chi2 = 9.318, P = 0.025). Logistic multivariate analysis showed that only the pathological type was associated with EGFR expression (P = 0.047). The p-Akt expression was not related with the above clinicopathological features (P > 0.05) Kaplan-Meire survival analysis showed that neither of the two proteins had an impact on the patients' survival (P = 0.972 and P = 0.903 respectively). CONCLUSION: Protein expressions of IEGFR and p-Akt have no impact on NSCLC patients' survival, thus so far they can not serve as molecular staging or prognostic indicators.