| 应用双重原位杂交技术定位检测肝细胞癌中乙型,丙型肝炎病毒 |
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| 引用本文： | 刘彦君,丛文铭.应用双重原位杂交技术定位检测肝细胞癌中乙型,丙型肝炎病毒[J].第二军医大学学报,1996,17(2):105-108. |
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| 作者姓名： | 刘彦君 丛文铭 |
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| 作者单位： | 第二军医大学长海医院肝胆外科研究所肿瘤免疫和基因治疗中心 |
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| 摘 要： | 探索乙型肝炎病毒和丙型肝炎病毒是否可同时感染同一宿主细胞。联合应用地高辛标记探针和生物素标记探针的双标记方法,成功建立起非放射性双重原位杂交技术,并应用这一技术检测了１０例肝细胞性肝癌及癌周细胞细胞内乙型肝炎病毒和丙型肝炎病毒的感染情况。
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| 关 键 词： | 原位杂交 肝肿瘤 乙型肝炎病毒 丙型炎病毒 |
Detection of localization of hepatitis virus B and C in hepatocellular carcinomas by double in situ hybridization technique |
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| Liu Yanjun,Cong Wenming,Zhang Xiuzhong,Xie Tianpei,Shen Feng,Guo Yajun,Chen Han,Wu Mengchao.Detection of localization of hepatitis virus B and C in hepatocellular carcinomas by double in situ hybridization technique[J].Academic Journal of Second Military Medical University,1996,17(2):105-108. |
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| Authors: | Liu Yanjun Cong Wenming Zhang Xiuzhong Xie Tianpei Shen Feng Guo Yajun Chen Han Wu Mengchao |
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| Abstract: | Objective:To detect whether hepatitis B virus(HBV) and hepatitis C virus(HCV) could infect the same host cell.Methods:We established the method of non-isotope double in situ hybridization by combining digoxigenin-labeled probe with biotin-labeled probe,and detected infections of HBV and HCV in 10cases of hepatocellular carcinomas.Results:It was found that HBV and HCV could infect the same host cell,the specular biological phenomenon.Conclusion:Infections of HBV and HCV might not always obey the rule of "viral interference",providing a new clue for researching the synergistic action of HBV and HCV in the pathogenesis of hepatocellular carcinomas. |
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| Keywords: | in situ hybridization liver neoplasms hepatitis B virus hepatitis C virus |
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