AIDS患者胃黏膜CD4~+T淋巴细胞与HIV感染关系的初步分析

目的探讨AIDS患者胃黏膜CD4^＋T淋巴细胞的丢失、重建与HIV感染的关系。方法对35例AIDS患者胃黏膜组织冰冻切片和PBMC涂片采用核酸原位杂交方法及免疫组化双重染色,观察CD4^＋T淋巴细胞的HIV感染情况。结果①高效抗逆转录病毒治疗（HAART）治疗1～4年后,AIDS患者胃黏膜单个核细胞（MMC）中CD4^＋T细胞计数仍然低于对照组（37.44±18.00]%vs（50.35±3.41）%]（P〈0.01）;但PBMC中CD4^＋T细胞计数与对照组无显著性差异（42.70±10.66）%vs（51.00±6.60）%]（P〉0.05）。②未治疗组与治疗〈1年组胃黏膜MMC中CD4^＋T细胞HIV阳性率无显著性差异（2.76±1.92）%vs（1.88±1.56）%]（P〉0.05）;治疗1～4年组则明显低于未治疗组（0.54±0.49）%vs（2.76±1.92）%（P〈0.01）;三组AIDS患者PBMC中CD4^＋T细胞HIV感染率均具有显著性差异（19.44±8.17）%vs（9.76±6.98）%vs（4.95±8.26）%]（P〈0.05）。结论相对于外周血,AIDS患者胃黏膜免疫重建可能是不完全的。AIDS患者胃黏膜CD4^＋T细胞HIV感染在胃黏膜CD4^＋T细胞的丢失与重建延迟过程中可能并非发挥主要的作用。

The analysis for the relationship between the CD4~+T cell and HIV infection in gastric mucosa from patients with acquired immune deficiency syndrome

Objective To investigate the relationship between CD4~+T cell depletion and restoration and HIV infection in gastric mucosa from patients with AIDS. Methods 35 AIDS patients were enrolled.In situ hybridization (ISH) test and immunohistochemistry (IHC) method were performed to detect HIV infection in CD4~+T cells in gastric mucosal tissues patients had received HAART for 1～4 years,amount of CD4~+ T cells in MMC were lower than that of control group(37.44±18.001% vs (50.35±3.41)%](P<0.01);However,amount of CD4~+T cells in PBMC were similar with that of control group had not received HAART and AIDS patients with HAART for less than one year was not obviously different(2.76±1.92)%vs(1.88±1.56)%](P＞0.05).CD4~+T cells in the patients who had received HAART for 1～4 years were more depleted (0.54±0.49)%;Among 3 AIDS groups,we found the HIV infection of CD4~+T cells in PBMC was different (19.44±8.17)% vs(9.76±6.98)%vs(4.95±8.26)%,respectively](P<0.05). Conclusions It may indicate that CD4~+T cells restoration was delayed during HAART compared with peripheral blood ,HIV infection in CD4~+T cell may not produce a marked effect in the depletion and delayed restoration of CD4~+T cell in gastric mucosa from patients with AIDS.