芩丹颗粒对微波辐射损伤大鼠信号转导影响

目的探讨芩丹颗粒对微波辐射损伤大鼠的防护作用。方法制作微波辐射大鼠模型,检测微波辐射前后体温变化,用芩丹颗粒进行干预,检测各组大鼠血清环化腺苷酸(cAMP)、蛋白激酶A(PKA)、促肾上腺激素(ACTH)、β-内啡肽(β-EP)、热休克蛋白70(Hsp 70)。结果微波辐射后体温明显高于辐射前(P<0.05);微波辐射后1 d,辐射模型组血清中各指标与空白对照组无明显差别(P>0.05);照射后2周,辐射模型组cAMP、β-EP、ACTH、PKA、Hsp 70分别为(23.49±8.96)nmol/L、(12.68±2.21),(86.39±28.88)pg/mL、(4.94±1.21)、(1.73±0.27)ng/mL,明显高于空白对照组(P<0.05),芩丹颗粒组cAMP(10.09±1.03)nmol/L、PKA(2.62±0.55)ng/mL、β-EP(4.61±0.69)pg/mL、Hsp 70(1.63±0.20 ng/mL)明显低于辐射模型组(P<0.05)。结论芩丹颗粒可通过调节cAMP、PKA、β-EP、ACTH含量对微波辐射的损伤起到防护作用。

Effect of Qindan particles on signal transduction in rats with microwave radiation damage

ObjectiveTo research protective effect of Qindan particles on microwave radiation damage.MethodsThe model of microwave radiation in rat was established.The body temperature change of the rats was detected before and after the radiation.After treated with Qindan particles,the serum cyclic adenosine-3'-5'monophosphate(cAMP),protein kinase A(PKA),adreno-cortic-tropic hormone(ACTH),β-endophin(β-EP),and heat shock protein 70(Hsp70)were detected.ResultsThe body temperature of the rats was obviously higher after microwave radiation(P<0.05).One day after the microwave radiation,cAMP,PKA,ACTH,β-EP and Hsp70 of microwave radiation model group were not obviously different from those of the control group(P>0.05 for all).Two weeks after the radiation,cAMP(23.49±8.96 nmol/L),PKA (4.94±1.21 ng/mL),β-EP(12.68±2.21 pg/mL),ACTH(86.39±28.88 pg/mL),and Hsp70(1.73±0.27 ng/mL)of microwave radiation model group were significantly different from those of the control group(P<0.05 for all).The contents of cAMP(10.09±1.03 nmol/L),PKA(2.62±0.55 ng/mL),β-EP(4.61±0.69 pg/mL),and sHsp70(1.63±0.20 ng/mL)of Qindan particles group decreased obviously compared to those of microwave radiation model group(P<0.05 for all).ConclusionQindan particles may protect the rats from microwave radiation damage by regulating serum cAMP, PKA,β-EP,and ACTH.