| 军团菌外膜蛋白抗原基因的克隆并在原核系统中表达 |
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| 引用本文： | 芦殿香,陈建平,张雷,王涛,刘明杰,田玉,陈宪. 军团菌外膜蛋白抗原基因的克隆并在原核系统中表达[J]. 寄生虫病与感染性疾病, 2004, 2(4): 149-151 |
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| 作者姓名： | 芦殿香 陈建平 张雷 王涛 刘明杰 田玉 陈宪 |
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| 作者单位： | 四川大学华西基础医学与法医学院寄生虫学教研室,形态学实验室,四川,成都,610041 |
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| 基金项目： | 国家自然科学基金 (编号 :3 0 3 0 0 3 0 2 )，四川省学术技术带头人培养基金 (编号 :42 0 0 3 16)资助课题。 |
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| 摘 要： | 目的克隆军团菌外膜蛋白抗原基因ompM，构建重组质粒pLPM，并在原核系统中表达。方法采用聚合酶链反应(PCR)从军团菌基因组DNA中扩增得到ompM基因，导入载体pUC18，在JM109中表达，并用SDS-PAGE进行鉴定。结果扩增出773bp的ompM基因；构建重组质粒pLPM；表达出25kDa的蛋白质。结论成功扩增军团菌ompM基因，构建重组质粒pLPM，并在原核系统中得到了表达。
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| 关 键 词： | 军团菌 ompM PCR 基因表达 |
| 文章编号： | 1672-2116(2004)04-0149-03 |
| 修稿时间： | 2004-10-25 |
Cloning of Major Outer Membrane Protein Antigen Gene of Legionella pneumophila and Detection of Its Expression in Prokaryotic Cell |
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| LU Dian-xiang,CHEN Jian-ping,ZHANG Lei,WANG Tao,LIU Ming-jie,TIAN Yu,CHEN Xian. Cloning of Major Outer Membrane Protein Antigen Gene of Legionella pneumophila and Detection of Its Expression in Prokaryotic Cell[J]. Parastoses and Infectious Diseases, 2004, 2(4): 149-151 |
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| Authors: | LU Dian-xiang CHEN Jian-ping ZHANG Lei WANG Tao LIU Ming-jie TIAN Yu CHEN Xian |
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| Affiliation: | LU Dian-xiang,CHEN Jian-ping*,ZHANG Lei,WANG Tao,LIU Ming-jie,TIAN Yu,CHEN Xian |
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| Abstract: | Objective To clone the major outer membrane antigen gene omp M of Legionella pneumophila, to construct recombinant plasmid pLPM and to dete ct its expre ssion in prokaryotic cell strain JM109.Methods The ompM gene fr o m Legionella pneumophila was amplified with PCR. The PCR product was cloned into pUC18 vector a nd ompM expressed in JM109. After that, the expression of JM109 of ompM gene was det ected with SDS-PAGE. Results The ompM gene of 773bp was amplif ied, the recombin ant plasmid pLPM constructed and protein of 25 kDa expressed in JM109.C onclusion The ompM gene was amplified, the recombinant plasmid pLPM cons tructed and its expression detected in prokaryotic cell successfully. |
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| Keywords: | Legionella pneumophila ompM PCR gene expression |
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