| 硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞免疫功能的影响 |
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| 引用本文： | 刘佳,潘道东. 硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞免疫功能的影响[J]. 营养学报, 2013, 35(1): 35-38 |
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| 作者姓名： | 刘佳 潘道东 |
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| 作者单位： | 1. 宁波大学海洋学院食品系,宁波,315211
2. 南京师范大学食品系,南京,210097 |
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| 基金项目： | 国家自然科学基金(No.30972130);浙江省自然基金重点项目(No.Z3110211);宁波市重大攻关项目(No.2011C11017) |
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| 摘 要： | 目的探讨硒化乳酸菌胞外多糖(Se-EPS)对小鼠腹腔巨噬细胞免疫活性的影响。方法从乳酸菌培养液中分离提纯乳酸菌胞外多糖(EPS),并将EPS与无机硒化剂反应,得到Se-EPS。采用体外细胞培养,测定Se-EPS对巨噬细胞吞噬能力,胞外一氧化氮(NO)与肿瘤坏死因子(TNF-α)分泌量的影响。结果 Se-EPS能够显著增强巨噬细胞的吞噬活性,NO和TNF-α的分泌量,并且在Se-EPS浓度为50～1000 g/ml之间呈浓度依赖性增加。结论 Se-EPS有助于促进巨噬细胞的细胞介导免疫反应、增强免疫反应的作用。
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| 关 键 词： | 乳酸菌胞外多糖 硒化 巨噬细胞 免疫活性 |
THE IMMUNOMODULATORY EFFECTS OF SELENIUM EXOPOLYSACCHARIDE ON MOUSE PERITONEAL MACROPHAGES |
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| LIU Jia,PAN Dao-dong. THE IMMUNOMODULATORY EFFECTS OF SELENIUM EXOPOLYSACCHARIDE ON MOUSE PERITONEAL MACROPHAGES[J]. Acta Nutrimenta Sinica, 2013, 35(1): 35-38 |
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| Authors: | LIU Jia PAN Dao-dong |
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| Affiliation: | 1(Food Science & Engineering Department of Marine School,Ningbo University,Ningbo 315211;1Food Science Department of Nanjing Normal University,Nanjing 210097,China) |
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| Abstract: | Objective The immunomodulating effects of selenium exopolysaccharide(Se-EPS) on mouse peritoneal macrophages were investigated.Method Expolysaccharides(EPS),obtained from lactic acid bacteria culture,were isolated and purified,then reacted with inorganic selenium to form Se-EPS.The effects of Se-EPS on the phagocytic capability,nitrous oxide(NO) release and tumor necrosis factor-α(TNF-α) activity of mouse peritoncal macrophages were assayed in vitro.Results Se-EPS,from 50 to 1 000 μg/ml,could enhance macrophages phagocytosis and increase the production of NO and TNF-α in dose-dependent manner.Conclusion Se-EPS could promote macrophage-mediated immune reaction and boost immune response. |
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| Keywords: | exopolysaccharide selenium macrophage immunomodulatory effect |
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